Effect of polishing and immersion in disinfectant solutions on the physical and biological properties of denture base resin for 3D printing

Abstract

In recent years, there has been a trend towards replacing conventional impressions and plaster models with images obtained from intraoral scanning for the manufacture of partial and/or total dentures. However, this technique does not reduce the formation of biofilm on the materials used, enabling the occurrence of fungal diseases such as prosthetic stomatitis. Thus, the aim of this in vitro study will be to evaluate the effect of polishing and immersion times in disinfectant solutions on physical (color stability, hardness, roughness, surface free energy and surface topography) and biological (adhesion and surface topography) properties. Biofilm formation and cytotoxicity) of resin obtained through 3D printing. Samples (14 mm in diameter and 1.2 mm thick) will be projected virtually and will be made in a 3D printer. After making, the samples will be divided into 2 groups according to the polishing protocol: CP: samples obtained through 3D printing will undergo a polishing protocol and will be stored in distilled water for 48 hours; SP: samples obtained through 3D printing will not be polished and will be stored in distilled water for 48 hours. Samples from groups CP and SP will be subdivided into new groups, according to the disinfectant solutions, as follows: CPAD: polished samples immersed in distilled water; CPHS: polished samples immersed in 0.5% sodium hypochlorite; CPDC: polished samples immersed in 2% chlorhexidine gluconate; CPSL: polished samples immersed in a disinfectant liquid soap solution (Lifebuoy); SPAD: unpolished samples immersed in distilled water; SPHS: unpolished samples immersed in 0.5% sodium hypochlorite; SPDC: unpolished samples immersed in 2% chlorhexidine gluconate; SPSL: unpolished samples immersed in a disinfectant liquid soap solution (Lifebuoy). All samples will be immersed at immersion times of 0, 1, 3 and 6 months and after each period, the physical and biological properties of the samples will be evaluated. The results will be tabulated and submitted to the normality test to (AU)