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Priming effect of phothobiomodulation therapy on osteogenic potential of adipose tissue-derived mesenchymal stem cells

Grant number: 21/04874-1
Support type:Regular Research Grants
Duration: March 01, 2022 - February 29, 2024
Field of knowledge:Health Sciences - Dentistry - Oral and Maxillofacial Surgery
Principal researcher:Emanuela Prado Ferraz
Grantee:Emanuela Prado Ferraz
Home Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Assoc. researchers:Marcia Martins Marques ; Márcio Mateus Beloti ; Praveen Arany

Abstract

In order to improve bone regeneration, the use of mesenchymal stem cells (MSC) from different sources have been widely explored. MSCs from adipose tissue (MSC-AT) are an attractive alternative since a higher number of cells are easily harvested at lesser morbidity compared to cells obtained from other sources. However, their use is limited due to their lower less osteogenic potential. To enhance cell differentiation, photobiomodulation therapy (PBM) emerges as a non-invasive therapy that has been related to the acceleration of the repair process. In this context, the aim of this project is to investigate the PBM as a therapeutic alternative to enhance the osteoblastic differentiation of MSC-TA to be used as cell therapy in the repair of bone defects. For this, rats MSC-AT will be cultured in expansion or osteogenic medium and submitted to the PBM protocol (660 nm; 0.14 J; 20 mW; 0.714 W/cm2 and 5 J/cm2). The effect of PBM and the conditioned medium by MSCs exposed to PBM on osteoblastic differentiation of MSC-AT will be evaluated by gene and protein expression of bone markers, ALP activity and production of mineralized extracellular matrix. The conditioned medium will be characterized for the expression of a panel of proteins. Bone defects on rat calvaria will be filled with MSC-AT exposed to PBM or conditioned medium by MSC-AT exposed to PBM, and the repair will be evaluated using microtomographic and histological analysis. Depending on the adherence to the normal curve, the appropriate statistical test will be selected, and the level of significance will be 5%. The results of this study may contribute to the understanding of the intracellular mechanisms involved in the differentiation and activity of differentiated cells from MSC-AT and related to the PBM therapy, optimizing bone repair. (AU)

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