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Multi-user Equipament approved in grant 2019/11820-5: ChemiDoc Imaging System and accessories

Grant number: 21/06291-3
Support Opportunities:Multi-user Equipment Program
Duration: September 01, 2021 - August 31, 2028
Field of knowledge:Health Sciences - Physical Education
Principal Investigator:Adelino Sanchez Ramos da Silva
Grantee:Adelino Sanchez Ramos da Silva
Host Institution: Escola de Educação Física e Esporte de Ribeirão Preto (EEFERP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated research grant:19/11820-5 - Nr1d1 function on the aging-associated Sarcopenia, AP.TEM
As informações de acesso ao Equipamento Multiusuário são de responsabilidade do Pesquisador responsável
EMU web page: Página do Equipamento Multiusuário não informada
Type of equipment:Processos Biológicos - Caracterização - Leitores de fluorescência (imageamento, placas)
Processos Biológicos - Caracterização - Leitores de placas
Manufacturer: Fabricante não informado
Model: Modelo não informado

Abstract

Recently, the World Health Organization classified sarcopenia as a disease, which will allow the establishment of universal clinical guidelines for its diagnosis and treatment. In this sense, the rev-erb-alpha protein is a promising molecular target for the prevention and treatment of sarcopenia, since it plays an essential role in the regulation of autophagy, mitochondrial content, oxidative function, and skeletal muscle mass maintenance. Therefore, the present thematic project will investigate the function of the gene nuclear receptor subfamily 1 group D member 1 (Nr1d1) in the development of sarcopenia associated with aging through experiments on cells, rodents, and humans. For this, the following subprojects will be used: 1) Effect of aging on Nr1d1 and its relationship with sarcopenia; 2) Effect of global or conditional deletion of Nr1d1 on aging; 3) Interactome analysis of rev-erb-alpha protein through immunoprecipitation of the target protein and identification of possible ligands by mass spectrometry in cell culture; 4) Effects of pharmacological, nutritional and genetic activation of Nr1d1 on the aging process; 5) Effects of physical exercise on the aging-induced sarcopenia: the role of rev-erb-alpha in the skeletal muscle. The methodologies used in this project will be RNA-seq, immunoblotting, Real time-RTqPCR, interactome, mass spectrometry, gene therapy (CRISPR/Cas9 and adeno-associated virus vector), histology, immunohistochemistry, immunofluorescence, bioinformatics, and lysosomal autophagy flow. According to the statistical distribution of the data, parametric or non-parametric tests will be used to compare the studied parameters between the experimental groups. (AU)

Articles published in Agência FAPESP Newsletter about the research grant:
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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MARAFON, BRUNO B.; PINTO, ANA P.; DE VICENTE, LARISSA G.; DA ROCHA, ALISSON L.; SIMABUCO, FERNANDO M.; ROPELLE, EDUARDO R.; DE MOURA, LEANDRO P.; CINTRA, DENNYS E.; PAULI, JOSE R.; DA SILVA, ADELINO S. R.. Genetic ablation of Toll-like Receptor 4 seems to activate the apoptosis pathway in the skeletal muscle of mice after acute physical exercise. Cell Biochemistry and Function, v. N/A, p. 12-pg., . (20/13443-1, 20/04269-8, 19/11820-5, 21/06291-3)
PINTO, ANA P.; MUNOZ, VITOR R.; TAVARES, MARIA EDUARDA A.; DOS SANTOS, JONATHAS R.; REBELO, MACARIO A.; ALBERICI, LUCIANE C.; SIMABUCO, FERNANDO M.; TEIXEIRA, GIOVANA R.; PAULI, JOSE R.; DE MOURA, LEANDRO P.; et al. Combined physical exercise reverses the reduced expression of Bmal1 in the liver of aged mice. Life Sciences, v. 312, p. 12-pg., . (21/08693-1, 21/08692-5, 20/13443-1, 21/06291-3, 19/11820-5)
PINTO, ANA P.; MUNOZ, VITOR R.; DA ROCHA, ALISSON L.; ROVINA, RAFAEL L.; FERRARI, GUSTAVO D.; ALBERICI, LUCIANE C.; SIMABUCO, FERNANDO M.; TEIXEIRA, GIOVANA R.; PAULI, JOSE R.; DE MOURA, LEANDRO P.; et al. IL-6 deletion decreased REV-ERB alpha protein and influenced autophagy and mitochondrial markers in the skeletal muscle after acute exercise. FRONTIERS IN IMMUNOLOGY, v. 13, p. 13-pg., . (21/06291-3, 21/08693-1, 19/11820-5, 17/12765-2, 19/21709-4, 21/08692-5, 20/13443-1, 18/14818-9)
OLIVEIRA, LUCIANA DA COSTA; MORAIS, GUSTAVO PAROSCHI; DE OLIVEIRA, FRANCIANE PEREIRA; MATA, MILENE MONTAVONI; VERAS, ALLICE SANTOS CRUZ; DA ROCHA, ALISSON LUIZ; ELIAS, LUCILA LEICO KAGOHARA; TEIXEIRA, GIOVANA RAMPAZZO; DE MORAES, CAMILA; CINTRA, DENNYS E.; et al. Intermittent fasting combined with exercise training reduces body mass and alleviates hypothalamic disorders induced by high-fat diet intake. JOURNAL OF NUTRITIONAL BIOCHEMISTRY, v. 119, p. 12-pg., . (16/17968-6, 20/08192-0, 19/17532-1, 17/25492-4, 19/21709-4, 21/06291-3)

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