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A novel antimicrobial drug-delivery coating for percutaneous implant devices


Antimicrobial coatings on percutaneous devices (PDs), such as dental implants and exposed pins, are considered an effective strategy to avoid infection progression that might result in PD loss. Our conducted preliminary research validated the tetracycline incorporation method into the layer-by-layer system, assembled by alternating polyelectrolyte layers of poly(acrylic acid) (PAA) and poly(l-lysine) (PLL), through the synthesis of tetracycline with anionic beta cyclodextrin (²-CD). The succeeded [PAA/PLL]10/TC/anionic ²-CD biomaterial coating showed to be readily tunable to different physiological scenarios, with a controlled release of the drug up to 30 days. Antibacterial effect of [PAA/PLL]10/TC/anionic ²-CD was confirmed against Staphylococcus aureus ATCC 25923. The exciting outcomes encouraged us to proceed with the studies and translate the power of antimicrobial multilayer systems in meaningful biomedical applications. Herein, taking into consideration the importance of tissue quality surrounding PDs and the need to protect the drug into the multilayers, in this project, we will focus on the development of acidic pH sensible film with methacrylic acid (MAA)-based vascular regenerative to cover [PAA/PLL]10/TC/anionic ²-CD and to promote vascularization and subsequent wound healing. A detailed multilayer coating characterization will be performed by different microscopy and spectroscopy approaches to probe physical and chemical properties. Coating stability of MAA-film on [PAA/PLL]10/TC/anionic ²-CD system will be confirmed under neutral and acidic pH, mimicking healthy and diseased/inflammatory environments, respectively. Moreover, the molecular and cellular mechanisms of interaction in the presence of MAA-film will be investigated to improve the therapeutic effect of layer-by-layer coating system. Further, in vitro microbiology experiments will be performed to uncover the broad-spectrum of TC/anionic ²-CD against different bacteria involved in implant infection. Additionally, we will further demonstrate the effect of the antimicrobial multilayer system on inflammatory signaling pathways and epithelial downgrowth to facilitate the advancement of regenerative biomaterials for diverse applications, using in vivo model. (AU)

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Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MALHEIROS, SAMUEL S.; NAGAY, BRUNA E.; BERTOLINI, MARTINNA M.; DE AVILA, ERICA D.; SHIBLI, JAMIL A.; SOUZA, JOAO GABRIEL S.; BARAO, VALENTIM A. R.. Biomaterial engineering surface to control polymicrobial dental implant-related infections: focusing on disease modulating factors and coatings development. Expert Review of Medical Devices, v. 20, n. 7, p. 17-pg., . (21/09434-0, 22/15677-5, 20/05231-4, 18/20719-3, 19/17238-6)
VAN OIRSCHOT, BART A. J. A.; ZHANG, YANG; ALGHAMDI, HAMDAN S.; CORDEIRO, JAIRO M.; NAGAY, BRUNA E.; BARAO, VALENTIM A. R.; DE AVILA, ERICA DORIGATTI; VAN DEN BEUCKEN, JEROEN J. J. P.. Surface Engineering for Dental Implantology: Favoring Tissue Responses Along the Implant. TISSUE ENGINEERING PART A, v. 28, n. 11-12, p. 18-pg., . (20/05231-4, 18/20719-3, 21/09434-0, 19/17238-6, 17/01320-0, 15/03567-7)
DE MOLON, RAFAEL SCAF; ALVES PEREIRA, MARTA MARIA; DE AVILA, ERICA DORIGATTI. p Implant surface features as key role on cell behavior. BIOCELL, v. 46, n. 5, p. 1151-1156, . (18/20719-3)
MIRANDA, LUIS FERNANDO B.; LIMA, CAROLINA V.; PAGIN, RAFAELA; COSTA, RAPHAEL C.; PEREIRA, MARTA MARIA A.; DE AVILA, ERICA D.; BERTOLINI, MARTINNA; RETAMAL-VALDES, BELEN; SHIBLI, JAMIL A.; FERES, MAGDA; et al. Effect of Processing Methods of Human Saliva on the Proteomic Profile and Protein-Mediated Biological Processes. JOURNAL OF PROTEOME RESEARCH, v. 22, n. 3, p. 14-pg., . (21/09434-0, 18/20719-3, 20/10436-4, 21/10762-1)
DE AVILA, ERICA D.; NAGAY, BRUNA E.; PEREIRA, MARTA M. A. .; BARAO, VALENTIM A. . R. .; PAVARINA, ANA CLAUDIA; VAN DEN BEUCKEN, JEROEN J. J. P.. Race for Applicable Antimicrobial Dental Implant Surfaces to Fight Biofilm-Related Disease: Advancing in Laboratorial Studies vs Stagnation in Clinical Application. ACS BIOMATERIALS SCIENCE & ENGINEERING, v. 8, n. 8, p. 12-pg., . (21/09434-0, 18/20719-3, 20/05231-4)

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