A novel antimicrobial drug-delivery coating for percutaneous implant devices

Abstract

Antimicrobial coatings on percutaneous devices (PDs), such as dental implants and exposed pins, are considered an effective strategy to avoid infection progression that might result in PD loss. Our conducted preliminary research validated the tetracycline incorporation method into the layer-by-layer system, assembled by alternating polyelectrolyte layers of poly(acrylic acid) (PAA) and poly(l-lysine) (PLL), through the synthesis of tetracycline with anionic beta cyclodextrin (²-CD). The succeeded [PAA/PLL]10/TC/anionic ²-CD biomaterial coating showed to be readily tunable to different physiological scenarios, with a controlled release of the drug up to 30 days. Antibacterial effect of [PAA/PLL]10/TC/anionic ²-CD was confirmed against Staphylococcus aureus ATCC 25923. The exciting outcomes encouraged us to proceed with the studies and translate the power of antimicrobial multilayer systems in meaningful biomedical applications. Herein, taking into consideration the importance of tissue quality surrounding PDs and the need to protect the drug into the multilayers, in this project, we will focus on the development of acidic pH sensible film with methacrylic acid (MAA)-based vascular regenerative to cover [PAA/PLL]10/TC/anionic ²-CD and to promote vascularization and subsequent wound healing. A detailed multilayer coating characterization will be performed by different microscopy and spectroscopy approaches to probe physical and chemical properties. Coating stability of MAA-film on [PAA/PLL]10/TC/anionic ²-CD system will be confirmed under neutral and acidic pH, mimicking healthy and diseased/inflammatory environments, respectively. Moreover, the molecular and cellular mechanisms of interaction in the presence of MAA-film will be investigated to improve the therapeutic effect of layer-by-layer coating system. Further, in vitro microbiology experiments will be performed to uncover the broad-spectrum of TC/anionic ²-CD against different bacteria involved in implant infection. Additionally, we will further demonstrate the effect of the antimicrobial multilayer system on inflammatory signaling pathways and epithelial downgrowth to facilitate the advancement of regenerative biomaterials for diverse applications, using in vivo model. (AU)