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Cellular senescence in healthy and diseased periodontal tissues of smokers

Grant number: 20/03158-8
Support type:Regular Research Grants
Duration: July 01, 2021 - June 30, 2023
Field of knowledge:Health Sciences - Dentistry - Periodontology
Principal researcher:Tamires Szeremeske de Miranda
Grantee:Tamires Szeremeske de Miranda
Home Institution: Universidade Universus Veritas Guarulhos (Univeritas UNG). Guarulhos , SP, Brazil
Assoc. researchers: Hélio Doyle Pereira da Silva


Studies have demonstrated that cellular activities can be modulated or changed throughout life, giving rise to a cell profile recognized as senescent. Senescent cells have been related to several diseases and type of cancers. Smoking is known to induce cell senescence and early biological aging. In addition, it is recognized that smoking can modify the course of periodontitis, increasing the risk of disease development and worsening its progress. However, important questions about the biological mechanisms by which tobacco consumption affects the course of periodontal diseases remain so far. In this exploratory study, we suggest the hypothesis that cellular senescence may play a role in the pathogenesis of smoking-related periodontitis. Therefore, the aim of this study will be to compare cellular senescence markers in the gingival tissues of smokers and non-smokers, with and without periodontitis. 120 participants will be distributed into one of the following groups: SP (n = 30): Smokers with periodontitis (periodontitis stages III or IV, grade C, generalized); PHS (n = 30): Periodontally healthy smokers; NSP (n = 30): Non-smokers with periodontitis (periodontitis stages III or IV, grade C, generalized); PHNS (n = 30): Periodontally healthy non-smokers. Biopsies of gingival tissue from areas representative of the periodontal inflammatory process or periodontal health will be collected. The following evaluations will be performed in the gingival tissues: gene expression of p16INK4a, p53 and p14ARF by real-time PCR, activity of the enzyme ²-galactosidase, quantification of lipofuscin by means of histochemistry, quantification of the components of the senescence-associated secretory phenotype (SASP) (HMGB1, IL-6, IL-8, IL-1alfa, IL-1², TNF-alfa, MCP-1, MIP-1alfa, GRO-alfa, GRO-², MMP-1, MMP-3 , G-CSF and IGF-1) and cell signaling components related to human DNA damage and genotoxicity (ATR, ChK 1 and 2, H2A.X and p53) through multiplex immunoassay, and the total and phosphorylated NF- KB (p65) and p38MAPK by ELISA. Appropriate statistical tests will be used for comparison between groups, according to the normality of the data. A significance level of 5% will be used for all analyses. (AU)

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