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The role of essential amino acids in the de novo milk fat synthesis

Grant number: 20/16572-7
Support Opportunities:Regular Research Grants
Duration: June 01, 2021 - May 31, 2023
Field of knowledge:Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding
Principal Investigator:Bruno Biagioli
Grantee:Bruno Biagioli
Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
Associated researchers: Mark Daniel Hanigan


Currently, milk pricing is based on milk volume and quality. Milk fat is one of the most important milk components considered in the establishment of the final milk price. Consequently, researchers intend to better understand the effect of nutrition on the milk fat synthesis in the mammary gland. Recent studies showed that amino acid supplementation in diets of dairy cows affects milk fat yield and this effect have been related to the role that amino acids play in regulating the action of the mammalian target of rapamycin complex 1 (mTORC1), which is related to sterol regulatory-element-binding protein 1 (SREBP-1), an important transcription factor involved in regulating de novo fatty acid synthesis in mammary gland cells. However, this hypothesis linking amino acids and SREBP-1 has not always been confirmed, which in turn may be related to the interaction among amino acids, as well as amino acid supplementation levels. Thus, the objective of this study is to evaluate the effects and doses of essential amino acids in de novo milk fat synthesis in bovine mammary epithelial cells, at physiological levels. The study will involve two experiments using isotopically labelled acetate (13C-acetato) as a tracer. In the first experiment we will evaluate the individual effect of essential amino acids in de novo fatty acid synthesis in bovine mammary epithelial cells subjected to 12 treatments varying the amino acid profile. According to the results of the first experiment, we will select up to 4 amino acids that will affect de novo fatty acid synthesis the most and then we will evaluate doses of these amino acids in de novo synthesis of milk fat in bovine mammary gland epithelial cells. Understanding the link between amino acids and milk fat synthesis in the mammary gland has practical application in the diet formulation of dairy cows. (AU)

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