Research Grants 20/12037-0 - Aves selvagens, Mamíferos - BV FAPESP
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Diversity of piroplasmids in wild birds and mammals from Brazil

Grant number: 20/12037-0
Support Opportunities:Regular Research Grants
Start date: June 01, 2021
End date: May 31, 2023
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
Principal Investigator:Marcos Rogério André
Grantee:Marcos Rogério André
Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
Associated researchers:Darci Moraes Barros-Battesti ; Rosangela Zacarias Machado

Abstract

Piroplasmids are tick-borne apicomplexan protozoa that comprise Babesia, Rangelia, Cytauxzoon and Theileria genera. There is a wide diversity of species that affect different taxa of mammals and birds, which can cause diseases in animals and humans. Studies aiming to detect piroplasmids in the Brazilian fauna, which is extremely diverse, show that different and new species of these protozoa circulate in the country. The present study aims to investigate the occurrence and determine the genetic diversity of piroplasmids in different taxonomic groups of vertebrates in Brazil. For this purpose, DNA aliquots will be extracted from 2,494 biological samples from wild animals and associated ectoparasites (for some of them) belonging to several taxonomic groups (327 Pilosa, 27 Cingulata, 878 Chiroptera, 288 Artiodactyla, 219 Carnivora, 129 Rodentia, oito Didelphimorphia, 118 Perissodactyla e 500 Passeriformes) from different states all over Brazil. The biological samples will be submitted to PCR assays based on the 18S rRNA, hsp-70, cox-1, cox-3, cytB, B-tubulin genes, and ITS-1 and ITS-2 intergenic regions. Positive samples will be purified and sequenced by the Sanger method. Samples with multiple peaks in the chromatograms will be subjected to gene cloning. The phylogenetic positioning of the obtained sequences will be performed by dendogram construction. Finally, the alignment of the sequences will be used to calculate the diversity of nucleotides and the level of polymorphisms for the analysis of the genetic diversity of the agents. The genealogy of the nucleotide sequences will be performed using the Splitstree program. The present study will shed light on the diversity of piroplasmids among Brazilian wild fauna. (AU)

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Scientific publications (16)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE MELLO, VICTORIA VALENTE CALIFRE; PLACA, ANA JULIA VIDAL; LEE, DANIEL ANTONIO BRAGA; FRANCO, ELIZ OLIVEIRA; LIMA, LUCIANA; TEIXEIRA, MARTA M. G.; HEMSLEY, CLAUDIA; TITBALL, RICHARD W.; MACHADO, ROSANGELA ZACARIAS; ROGERIO-ANDRE, MARCOS. Molecular detection of blood-borne agents in vampire bats from Brazil, with the first molecular evidence of Neorickettsia sp. in Desmodus rotundus and Diphylla ecaudata. Acta Tropica, v. 244, p. 9-pg., . (20/12037-0)
COSTA OLIVEIRA BRAGA, MARIA DO SOCORRO; COSTA, FRANCISCO BORGES; CALCHI, ANA CLAUDIA; DE MELLO, VICTORIA VALENTE CALIFRE; MONGRUEL, ANNA CLAUDIA BAUMEL; DIAS, CLARA MORATO; BASSINI-SILVA, RICARDO; SILVA, ELLAINY MARIA CONCEICAO; PEREIRA, JOSE GOMES; RIBEIRO, LARISSA SARMENTO DOS SANTOS; et al. Molecular detection and characterization of vector-borne agents in common opossums (Didelphis marsupialis) from northeastern Brazil. Acta Tropica, v. 244, p. 14-pg., . (20/07826-5, 20/12037-0, 20/11755-6)
PERLES, L.; BARRETO, W. T. G.; DE MACEDO, G. C.; CALCHI, A. C.; BEZERRA-SANTOS, M.; MENDOZA-ROLDAN, J. A.; OTRANTO, D.; HERRERA, H. M.; BARROS-BATTESTI, D. M.; MACHADO, R. Z.; et al. Molecular detection of Babesia spp. and Rickettsia spp. in coatis (Nasua nasua) and associated ticks from midwestern Brazil. Parasitology Research, v. 122, n. 5, p. 8-pg., . (18/02753-0, 20/12037-0, 19/15150-4)
DE OLIVEIRA, AGATHA FERREIRA XAVIER; CALCHI, ANA CLAUDIA; STOCCO, ANIELI VIDAL; STOCCO, NAIARA VIDAL; COSTA, ALEXANDRE CARVALHO; MUREB, ELISABETH NEVES; PIRES, JEFERSON ROCHA; GUIMARAES, ANDRESA; RAIMUNDO, JULIANA MACEDO; DE ALMEIDA BALTHAZAR, DANIEL; et al. Expanding the universe of Piroplasmids: morphological detection and phylogenetic positioning of putative novel piroplasmids in black-eared opossums (Didelphis aurita) from southeastern Brazil, with description of "South American Marsupialia Group" of Piroplasmida. Parasitology Research, v. 122, n. 7, p. 12-pg., . (20/12037-0)
GONCALVES, LUIZ RICARDO; PALUDO, GIANE; BISOL, TALITA BARCELOS; PERLES, LIVIA; DE OLIVEIRA, LARYSSA BORGES; DE OLIVEIRA, CAMILA MANOEL; DA SILVA, THIAGO MERIGHI VIEIRA; NANTES, WESLEY ARRUDA GIMENES; DUARTE, MATHEUS ALMEIDA; SANTOS, FILIPE MARTINS; et al. olecular detection of piroplasmids in synanthropic rodents, marsupials, and associated ticks from Brazil, with phylogenetic inference of a putative novel Babesia sp. from white-eared opossum (Didelphis albiventris. Parasitology Research, v. 120, n. 10, . (20/12037-0, 18/02753-0)
SANTANA, MATHEUS DE SOUZA; HOPPE, ESTEVAM GUILHERME LUX; CARRARO, PAULO EDUARDO; CALCHI, ANA CLAUDIA; DE OLIVEIRA, LARYSSA BORGES; DO AMARAL, RENAN BRESSIANINI; MONGRUEL, ANNA CLAUDIA BAUMEL; MACHADO, DALIA MONIQUE RIBEIRO; BURGER, KARINA PAES; BARROS-BATESTTI, DARCI MORAES; et al. Molecular detection of vector-borne agents in wild boars (Sus scrofa) and associated ticks from Brazil, with evidence of putative new genotypes of Ehrlichia, Anaplasma, and haemoplasmas. TRANSBOUNDARY AND EMERGING DISEASES, v. 69, n. 5, p. 24-pg., . (19/26403-0, 20/12037-0, 19/24726-7, 20/07826-5, 18/02753-0)
BAUMEL MONGRUEL, ANNA CLAUDIA; MEDICI, EMILIA PATRICIA; CANENA, ARIEL DA COSTA; CALCHI, ANA CLAUDIA; MACHADO, ROSANGELA ZACARIAS; ANDRE, MARCOS ROGERIO. Expanding the Universe of Hemoplasmas: Multi-Locus Sequencing Reveals Putative Novel Hemoplasmas in Lowland Tapirs (Tapirus terrestris), the Largest Land Mammals in Brazil. MICROORGANISMS, v. 10, n. 3, p. 22-pg., . (20/12037-0, 18/02753-0, 19/26403-0)
PERLES, LIVIA; HERRERA, HEITOR M.; BARRETO, WANESSA T. G.; DE MACEDO, GABRIEL C.; CALCHI, ANA C.; MACHADO, ROSANGELA Z.; ANDRE, MARCOS R.. Multi-Locus Sequencing Reveals Putative Novel Anaplasmataceae Agents, 'Candidatus Ehrlichia dumleri' and Anaplasma sp., in Ring-Tailed Coatis (Carnivora: Nasua nasua) from Urban Forested Fragments at Midwestern Brazil. MICROORGANISMS, v. 10, n. 12, p. 15-pg., . (20/12037-0, 18/02753-0, 19/15150-4)
BAUMEL MONGRUEL, ANNA CLAUDIA; MEDICI, EMILIA PATRICIA; CANENA, ARIEL DA COSTA; CALCHI, ANA CLAUDIA; PERLES, LIVIA; BALLA RODRIGUES, BIANCA CARDENAL; SOARES, JOAO FABIO; MACHADO, ROSANGELA ZACARIAS; ANDRE, MARCOS ROGERIO. Theileria terrestris nov. sp.: A Novel Theileria in Lowland Tapirs (Tapirus terrestris) from Two Different Biomes in Brazil. MICROORGANISMS, v. 10, n. 12, p. 15-pg., . (20/12037-0)
PERLES, L.; DE MACEDO, G. C.; BARRETO, W. T. G.; FRANCISCO, G., V; HERRERA, H. M.; BARROS-BATTESTI, D. M.; MACHADO, R. Z.; ANDRE, M. R.. Longitudinal dynamics and health impact of Hepatozoon procyonis (Apicomplexa: Hepatozoidae) on naturally infected ring-tailed coatis Nasua nasua (Carnivora: Procyonidae) from Midwestern Brazil. TICKS AND TICK-BORNE DISEASES, v. 13, n. 5, p. 11-pg., . (20/12037-0, 18/02753-0, 19/15150-4)
ANDRE, MARCOS ROGERIO; CALCHI, ANA CLAUDIA; FURQUIM, MARIA EDUARDA CHIARADIA; DE ANDRADE, ISABELA; ARANTES, PAULO VITOR CADINA; DE MELO LOPES, LARA CRISTINA; DEMARCHI, IURI KAUAN LINS DO NASCIMENTO; FIGUEIREDO, MAYRA ARAGUAIA PEREIRA; DE PAULA LIMA, CIRILO ANTONIO; MACHADO, ROSANGELA ZACARIAS. Molecular Detection of Tick-Borne Agents in Cats from Southeastern and Northern Brazil. PATHOGENS, v. 11, n. 1, . (20/12773-8, 19/10101-5, 19/19027-2, 18/02753-0, 19/26109-5, 20/07826-5, 20/12037-0)
DE OLIVEIRA, LARYSSA BORGES; CALCHI, ANA CLAUDIA; VULTAO, JULIANA GABOARDI; YOGUI, DEBORA REGINA; KLUYBER, DANILO; ALVES, MARIO HENRIQUE; JEAN DESBIEZ, ARNAUD LEONARD; DE SANTI, MARIELE; SOARES, ALINE GIROTTO; SOARES, JOAO FABIO; et al. Molecular investigation of haemotropic mycoplasmas and Coxiella burnetii in free-living Xenarthra mammals from Brazil, with evidence of new haemoplasma species. TRANSBOUNDARY AND EMERGING DISEASES, v. 69, n. 5, p. 15-pg., . (20/12037-0, 18/02753-0)
PERLES, LIVIA; BARRETO, WANESSA TEIXEIRA GOMES; DE MACEDO, GABRIEL CARVALHO; HERRERA, HEITOR MIRAGLIA; MACHADO, ROSANGELA ZACARIAS; ANDRE, MARCOS ROGERIO. Neorickettsia sp. in coatis (Nasua nasua) in Brazil. REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA, v. 32, n. 3, p. 4-pg., . (18/02753-0, 20/12037-0, 19/15150-4)
PERLES, LIVIA; BASSINI-SILVA, RICARDO; JACINAVICIUS, FERNANDO CASTRO; BARRETO, WANESSA TEIXEIRA GOMES; DE MACEDO, GABRIEL CARVALHO; MARTINS, FILIPE SANTOS; HERRERA, HEITOR MIRAGLIA; MACHADO, ROSANGELA ZACARIAS; BARROS-BATTESTI, DARCI MORAES; ANDRE, MARCOS ROGERIO. Contribution to the knowledge of Neotrichodectes (Nasuicola) pallidus (Piaget, 1880) (Phthiraptera: Trichodectidae). VETERINARY PARASITOLOGY- REGIONAL STUDIES AND REPORTS, v. 41, p. 6-pg., . (19/19853-0, 17/01416-7, 20/12037-0, 20/11755-6, 18/24667-8, 19/15150-4)
KRAWCZAK, FELIPE DA SILVA; CALCHI, ANA CLAUDIA; NEVES, LUCIANNE CARDOSO; DIAS, SARAH ALVES; DA SILVA, BIANCA BARBARA FONSECA; PAULA, WARLEY VIEIRA DE FREITAS; DE PAULA, LUIZA GABRIELLA FERREIRA; TAVARES, MARIANA AVELAR; PADUA, GRACIELLE TELES; DE LIMA, NICOLAS JALOWITZKI; et al. Phylogenetic Inferences Based on Distinct Molecular Markers Confirm a Novel Babesia Species (Babesia goianiaensis nov. sp.) in Capybaras (Hydrochoerus hydrochaeris) and Associated Ticks. MICROORGANISMS, v. 11, n. 8, p. 13-pg., . (20/07826-5, 20/12037-0)
PERLES, L.; OTRANTO, D.; BARRETO, W. T. G.; DE MACEDO, G. C.; LIA, R. P.; MENDOZA-ROLDAN, J. A.; HERRERA, H. M.; DE OLIVEIRA, C. E.; MACHADO, R. Z.; ANDRE, M. R.. Mansonella sp. and associated Wolbachia endosymbionts in ring-tailed coatis (Nasua nasua) in periurban areas from Midwestern Brazil. INTERNATIONAL JOURNAL FOR PARASITOLOGY-PARASITES AND WILDLIFE, v. 22, p. 6-pg., . (20/12037-0, 18/02753-0, 21/14244-5, 19/15150-4)

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