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Multi-user equipment approved in grant 2013/07937-8: Time Lapse

Grant number: 21/03972-0
Support Opportunities:Multi-user Equipment Program
Duration: June 01, 2021 - May 31, 2028
Field of knowledge:Biological Sciences - Biophysics - Radiology and Photobiology
Principal Investigator:Ohara Augusto
Grantee:Ohara Augusto
Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:13/07937-8 - Redoxome - Redox Processes in Biomedicine, AP.CEPID
As informações de acesso ao Equipamento Multiusuário são de responsabilidade do Pesquisador responsável
EMU web page: Página do Equipamento Multiusuário não informada
Type of equipment:Caracterização de Materiais - Microscopia ótica - Confocal
Caracterização de Materiais - Espectroscopia - Fluorescência (inclui resolvida no tempo)
Manufacturer: Fabricante não informado
Model: Modelo não informado


Timelapse is a time-resolved confocal fluorescence microscope system that can analyze a multitude of parameters down to the single molecule level using methods such as Fluorescence Lifetime Imaging (FLIM), FLIM/FRET, deep tissue FLIM, PIE, FCS/FCCS, FLCS/FLCCS, dual-focus FCS, scanning FCS, anisotropy, burst analysis, simultaneous AFM/FLIM or deep UV detection, to name only the most common. The advances of cutting-edge science in many fields depend on single molecule studies. This includes, for example, the quantification of molecular dynamics or molecular properties as well as interaction studies in material and life sciences. Such a wide field of research requires a flexible instrument, which can be adapted to the individual needs. Extension to the UVA region will allow fundamental applications for redox biochemistry for example to follow concentration profiles of NADH and FADH2 in cells as a function of time and location. (AU)

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