Multi-user equipment approved in grant 2013/07937-8: Time Lapse

Abstract

Timelapse is a time-resolved confocal fluorescence microscope system that can analyze a multitude of parameters down to the single molecule level using methods such as Fluorescence Lifetime Imaging (FLIM), FLIM/FRET, deep tissue FLIM, PIE, FCS/FCCS, FLCS/FLCCS, dual-focus FCS, scanning FCS, anisotropy, burst analysis, simultaneous AFM/FLIM or deep UV detection, to name only the most common. The advances of cutting-edge science in many fields depend on single molecule studies. This includes, for example, the quantification of molecular dynamics or molecular properties as well as interaction studies in material and life sciences. Such a wide field of research requires a flexible instrument, which can be adapted to the individual needs. Extension to the UVA region will allow fundamental applications for redox biochemistry for example to follow concentration profiles of NADH and FADH2 in cells as a function of time and location. (AU)