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Antitumor effect of Synadenium grantii Hook f. latex on preclinical models of mammary gland tumors

Grant number: 19/24079-1
Support type:Regular Research Grants
Duration: September 01, 2020 - August 31, 2022
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Pathology
Cooperation agreement: CONFAP - National Council of State Research Support Foundations
Principal researcher:Carlos Eduardo Fonseca Alves
Grantee:Carlos Eduardo Fonseca Alves
Home Institution: Vice-Reitoria de Pós-Graduação e Pesquisa. Universidade Paulista (UNIP). São Paulo , SP, Brazil
Assoc. researchers: Joelma Abadia Marciano de Paula ; Moema Pacheco Chediak Matos ; Renee Laufer Amorim

Abstract

Mammary tumors represent the most common neoplasms in dogs and women both. Similarities between the tumors in both species include spontaneous appearance, tumor evolution, biological behavior, and the expression different molecules. Thus, the dog is considered a natural model for the disease. Some tumors represent a therapeutic challenge and new therapies are needed for better patient prognosis. Different chemotherapeutic drugs were extracted from botanical compounds, and the Brazilian savanna presents a diversity of plants that may have potential for identification of new drugs. Synadenium grantii Hook f., a botanical species used in ethnomedicine in central-western and southern Brazil, is used empirically to treat a variety of diseases, including cancer. Thus, studies with S. grantii latex are promising for identification of antitumor compounds. Thus, the present research aims to evaluate chemical characteristics of the crude ethanolic extract (BSE) of Synadenium grantii Hook f. latex, to separate and identify active compounds by high performance liquid chromatography (HPLC) and high resolution mass spectrophotometry (EMAR). Also, to evaluate the effects of crude ethanolic extract of S. grantii latex in vitro and in vivo models of breast carcinoma. The HPLC will be performed to identify the compounds present in S. grantii latex, and their in vitro antitumor action will be evaluated in female and female mammary tumor cells. For this, the IC50 dose of S. grantii latex will be identified, and the cells will be treated to evaluate the effect of latex on cell migration and induction of apoptosis/necrosis. Subsequently, the in vivo antitumor effect will be evaluated on ET. Therefore, the in vivo TE model will be established to evaluate the antitumor effect of S. grantii latex. (AU)

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ROZOLEN, JULIANA MOREIRA; WICHERT TEODORO, TAMIRES GONELI; SOBRAL, RENATA AFONSO; RUIZ SUEIRO, FELIPE AUGUSTO; LAUFER-AMORIM, RENEE; ELIAS, FABIANA; FONSECA-ALVES, CARLOS EDUARDO. Investigation of Prognostic Value of Claudin-5, PSMA, and Ki67 Expression in Canine Splenic Hemangiosarcoma. ANIMALS, v. 11, n. 8 AUG 2021. Web of Science Citations: 0.
ANDRADE BRITO, ERIC SAYMOM; TABORDA PRADO, LAIS DI PAULIE; COSTA ARAUJO, LIANA KESIA; ARNHOLD, EMMANUEL; CHEDIAK MATOS, MOEMA PACHECO; MARCIANO DE PAULA, JOELMA ABADIA; RAMOS, LUCIANA MACHADO; FONSECA-ALVES, CARLOS EDUARDO; BRIANEZI DIGNANI DE MOURA, VERIDIANA MARIA. Effects of the Latex of Synadenium grantii Hook F. (Euphorbiaceae) on a Preclinical Model of Canine Prostate Cancer. FRONTIERS IN VETERINARY SCIENCE, v. 8, APR 12 2021. Web of Science Citations: 0.
LAINETTI, PATRICIA F.; LEIS-FILHO, ANTONIO F.; KOBAYASHI, PRISCILA E.; DE CAMARGO, LAIZA S.; LAUFER-AMORIM, RENEE; FONSECA-ALVES, CARLOS E.; SOUZA, FABIANA F. Proteomics Approach of Rapamycin Anti-Tumoral Effect on Primary and Metastatic Canine Mammary Tumor Cells In Vitro. Molecules, v. 26, n. 5 MAR 2021. Web of Science Citations: 0.
LAINETTI, PATRICIA DE FARIA; LEIS-FILHO, ANTONIO FERNANDO; LAUFER-AMORIM, RENEE; BATTAZZA, ALEXANDRE; FONSECA-ALVES, CARLOS EDUARDO. Mechanisms of Resistance to Chemotherapy in Breast Cancer and Possible Targets in Drug Delivery Systems. PHARMACEUTICS, v. 12, n. 12 DEC 2020. Web of Science Citations: 0.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.