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Development of a new thermosensitive hydrogel for controlled release of drugs to prevent and treat peri-implant inflammations


Thermosensitive hydrogels have been widely used in the biotechnology field as carriers for controlled drug release, and can be an alternative for the prevention and treatment of peri-implant inflammations. Thus, the present study aims to develop a new hydrogel based on poly (N-vinylcaprolactam) polymer for controlled release of tetracycline (TC) and evaluate its properties and composition, its antibacterial activity in vitro and in situ, as well as its interaction with cells in vitro and in vivo. Initially, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of TC will be determined to establish the initial drug concentration to be incorporated into the hydrogel. Thus, the following groups will be tested: (1) Hydrogel without the addition of TC as a control; (2) Hydrogel + TC at the concentration determined by MBC; (3) Hydrogel + TC with five times the concentration determined by MBC; and (4) Hydrogel + TC with ten times the concentration determined by MBC. Three studies will be conducted: in the study 1 in vitro the hydrogel in different concentrations of TC will be characterized in relation to morphology using scanning electron microscopy (SEM), molecular structure by Fourier transform infrared spectroscopy (FTIR), critical solution temperature by visible ultraviolet spectroscopy (UV/VIS), gelation time through the tilt method, swelling ratio by assessing liquid absorption, rheological properties by means of a rheometer and degradation through loss of mass after immersion in a solution containing the enzyme collagenase type II. TC release will be evaluated by high performance liquid chromatography (HPLC) after 1, 3, 7 and 15 days. The atomic force microscopy will be performed to evaluate how TC release occurs. The adhesion of the hydrogel to titanium will be analyzed by the shear test. The antimicrobial activity of the hydrogel will be evaluated in vitro using a microcosm model through the quantification of colony forming units (CFU/mL), microbial composition analysis by DNA-DNA checkerboard and structural analysis of the biofilm by SEM and confocal laser microscopy. Qualitative and quantitative analyzes will be performed to confirm the absence of toxicity of the hydrogel when in contact with human gingival fibroblast (HGF) cells. In the study 2 in situ, the most appropriate TC concentration according to the results of study 1 will be used to verify the antibacterial effectiveness of the hydrogel by quantifying CFU/mL, microbial composition analysis by DNA-DNA checkerboard and live and dead cells by confocal laser microscopy. Finally, the new material will be investigated regarding its effects on the inflammatory response and consequent influence on the healing process in an animal model. Herein, an implant will be installed at the tibia of each rat, in both sides, totaling two implants per animal. In order to mimic the infectious environment, the implants corresponding to the experimental group will be contaminated, in vitro, and the biofilm developed onto them will be removed by scaling, simulating a clinical stage, as mandatory step to be performed prior to the treatment proposed. Then, the hydrogel containing TC will be applied around the threads exposed by the bone defect, simulating bone resorption. Therefore, analyses to confirm the microbial viable colonies and microbial profile on the implants after treatment will be performed by CFU/mL, and DNA-DNA checkerboard, respectively. Additionally, the histopathological features of inflammation will be studied through histological analysis and the expression levels of inflammatory cytokines will be quantified by Multiplex technology. Quantitative data will be subjected to appropriate statistical analysis with a 5% significance level. (AU)

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Scientific publications (20)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MALHEIROS, SAMUEL S.; NAGAY, BRUNA E.; BERTOLINI, MARTINNA M.; DE AVILA, ERICA D.; SHIBLI, JAMIL A.; SOUZA, JOAO GABRIEL S.; BARAO, VALENTIM A. R.. Biomaterial engineering surface to control polymicrobial dental implant-related infections: focusing on disease modulating factors and coatings development. Expert Review of Medical Devices, v. 20, n. 7, p. 17-pg., . (21/09434-0, 22/15677-5, 20/05231-4, 18/20719-3, 19/17238-6)
DINI, CAROLINE; BORGES, GUILHERME ALMEIDA; COSTA, RAPHAEL CAVALCANTE; MAGNO, MARCELA BARAUNA; MAIA, LUCIANNE COPLE; RICARDO BARAO, VALENTIM ADELINO. Peri-implant and esthetic outcomes of cemented and screw-retained crowns using zirconia abutments in single implant-supported restorations-A systematic review and meta-analysis. Clinical Oral Implants Research, v. 32, n. 10, . (20/05231-4, 20/05234-3)
NAGAY, BRUNA EGUMI; DINI, CAROLINE; BORGES, GUILHERME ALMEIDA; MESQUITA, MARCELO FERRAZ; CAVALCANTI, YURI WANDERLEY; MAGNO, MARCELA BARAUNA; MAIA, LUCIANNE COPLE; RICARDO BARAO, VALENTIM ADELINO. Clinical efficacy of anodized dental implants for implant-supported prostheses after different loading protocols: A systematic review and meta-analysis. Clinical Oral Implants Research, v. 32, n. 9, . (20/05231-4, 19/17238-6)
COSTA, RAPHAEL C.; BERTOLINI, MARTINNA; COSTA OLIVEIRA, BARBARA E.; NAGAY, BRUNA E.; DINI, CAROLINE; BENSO, BRUNA; KLEIN, MARLISE I.; BARAO, VALENTIM A. R.; SOUZA, JOAO GABRIEL S.. Polymicrobial biofilms related to dental implant diseases: unravelling the critical role of extracellular biofilm matrix. CRITICAL REVIEWS IN MICROBIOLOGY, v. N/A, p. 21-pg., . (20/10436-4, 20/05231-4)
VALENTIM A. R. BARÃO; RAPHAEL C. COSTA; JAMIL A. SHIBLI; MARTINNA BERTOLINI; JOÃO GABRIEL S. SOUZA. Emerging titanium surface modifications: The war against polymicrobial infections on dental implants. Brazilian Dental Journal, v. 33, n. 1, p. 1-12, . (20/05231-4, 20/10436-4)
SAHA, SOUMYA; COSTA, RAPHAEL CAVALCANTE; SILVA, MIRELA CAROLINE; FONSECA-SANTOS, JOAO MATHEUS; CHEN, LIN; PHAKATKAR, ABHIJIT H.; BHATIA, HARSHDEEP; FAVERANI, LEONARDO P.; BARAO, VALENTIM A. R.; SHOKUHFAR, TOLOU; et al. Collagen membrane functionalized with magnesium oxide via room-temperature atomic layer deposition promotes osteopromotive and antimicrobial properties. BIOACTIVE MATERIALS, v. 30, p. 16-pg., . (22/16267-5, 20/05231-4, 20/10436-4)
FERES, MAGDA; MARTINS, RODRIGO; SOUZA, JOAO GABRIEL S.; BERTOLINI, MARTINNA; BARAO, VALENTIM A. R.; SHIBLI, JAMIL A.. Unraveling the effectiveness of antibiotics for peri-implantitis treatment: A scoping review. CLINICAL IMPLANT DENTISTRY AND RELATED RESEARCH, v. 25, n. 4, p. 15-pg., . (20/05231-4)
SCHELKOPF, STUART; DINI, CAROLINE; BELINE, THAMARA; WEE, ALVIN G.; BARAO, VALENTIM A. R.; SUKOTJO, CORTINO; YUAN, JUDY CHIA-CHUN. The Effect of Smoking and Brushing on the Color Stability and Stainability of Different CAD/CAM Restorative Materials. MATERIALS, v. 15, n. 19, p. 13-pg., . (20/05231-4, 20/05234-3)
VAN OIRSCHOT, BART A. J. A.; ZHANG, YANG; ALGHAMDI, HAMDAN S.; CORDEIRO, JAIRO M.; NAGAY, BRUNA E.; BARAO, VALENTIM A. R.; DE AVILA, ERICA DORIGATTI; VAN DEN BEUCKEN, JEROEN J. J. P.. Surface Engineering for Dental Implantology: Favoring Tissue Responses Along the Implant. TISSUE ENGINEERING PART A, v. 28, n. 11-12, p. 18-pg., . (20/05231-4, 18/20719-3, 21/09434-0, 19/17238-6, 17/01320-0, 15/03567-7)
BARA, VALENTIM A. R.; RAMACHANDRAN, REMYA AMPADI; MATOS, ADAIAS OLIVEIRA; BADHE, RAVINDRA VIJAYKUMAR; GRANDINI, CARLOS R.; SUKOTJO, CORTINO; OZEVIN, DIDEM; MATHEW, MATHEW. Prediction of tribocorrosion processes in titanium-based dental implants using acoustic emission technique: Initial outcome. Materials Science & Engineering C-Materials for Biological Applications, v. 123, . (20/05231-4)
SHAHMOHAMMADI, MINA; NAGAY, BRUNA E.; BARAO, VALENTIM A. R.; SUKOTJO, CORTINO; JURSICH, GREGORY; TAKOUDIS, CHRISTOS G.. Atomic layer deposition of TiO2, ZrO2 and TiO2/ZrO2 mixed oxide nanofilms on PMMA for enhanced biomaterial functionalization. Applied Surface Science, v. 578, p. 13-pg., . (20/05231-4, 19/17238-6)
SILVA SOUZA, JOAO GABRIEL; BERTOLINI, MARTINNA MENDONCA; COSTA, RAPHAEL CAVALCANTE; NAGAY, BRUNA EGUMI; DONGARI-BAGTZOGLOU, ANNA; RICARDO BARAO, VALENTIM ADELINO. Targeting implant-associated infections: titanium surface loaded with antimicrobial. ISCIENCE, v. 24, n. 1, . (20/05231-4)
COSTA, RAPHAEL C.; NAGAY, BRUNA E.; BERTOLINI, MARTINNA; COSTA-OLIVEIRA, BARBARA E.; SAMPAIO, ALINE A.; RETAMAL-VALDES, BELEN; SHIBLI, JAMIL A.; FERES, MAGDA; BARAO, VALENTIM A. R.; SOUZA, JOAO GABRIEL S.. Fitting pieces into the puzzle: The impact of titanium-based dental implant surface modifications on bacterial accumulation and polymicrobial infections. ADVANCES IN COLLOID AND INTERFACE SCIENCE, v. 298, . (20/05231-4, 20/10436-4)
DINI, CAROLINE; ALVES PEREIRA, MARTA MARIA; SILVA SOUZA, JOAO GABRIEL; SHIBLI, JAMIL A.; AVILA, ERICA DORIGATTI; BARAO, VALENTIM ADELINO R.. ssociation between industry support and the reporting of study outcomes in randomized clinical trials of dental implant research from the past 20 year. CLINICAL IMPLANT DENTISTRY AND RELATED RESEARCH, v. 24, n. 1, . (20/05234-3, 20/05231-4)
SILVA, JOAO PEDRO DOS S.; COSTA, RAPHAEL C.; NAGAY, BRUNA E.; BORGES, MARIA H. R.; SACRAMENTO, CATHARINA M.; DA CRUZ, NILSON C.; RANGEL, ELIDIANE C.; FORTULAN, CARLOS A.; DA SILVA, JOSE H. D.; RUIZ, KARINA G. S.; et al. Boosting Titanium Surfaces with Positive Charges: Newly Developed Cationic Coating Combines Anticorrosive and Bactericidal Properties for Implant Application. ACS BIOMATERIALS SCIENCE & ENGINEERING, v. 9, n. 9, p. 16-pg., . (20/05231-4)
DE AVILA, ERICA D.; NAGAY, BRUNA E.; PEREIRA, MARTA M. A. .; BARAO, VALENTIM A. . R. .; PAVARINA, ANA CLAUDIA; VAN DEN BEUCKEN, JEROEN J. J. P.. Race for Applicable Antimicrobial Dental Implant Surfaces to Fight Biofilm-Related Disease: Advancing in Laboratorial Studies vs Stagnation in Clinical Application. ACS BIOMATERIALS SCIENCE & ENGINEERING, v. 8, n. 8, p. 12-pg., . (21/09434-0, 18/20719-3, 20/05231-4)
RAMACHANDRAN, REMYA AMPADI; BARAO, VALENTIM A. R.; OZEVIN, DIDEM; SUKOTJO, CORTINO; PAI, P. SRINIVASA; MATHEW, MATHEW. Early predicting tribocorrosion rate of dental implant titanium materials using random forest machine learning models. TRIBOLOGY INTERNATIONAL, v. 187, p. 13-pg., . (20/05231-4)
ABDO, VICTORIA L.; SUAREZ, LINA J.; DE PAULA, LUCCA GOMES; COSTA, RAPHAEL C.; SHIBLI, JAMIL; FERES, MAGDA; BARA, VALENTIM A. R.; BERTOLINI, MARTINNA; SOUZA, JOAO GABRIEL SILVA. Underestimated microbial infection of resorbable membranes on guided regeneration. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 226, p. 16-pg., . (20/05231-4, 20/10436-4)
SOUZA, JOAO G. S.; COSTA, RAPHAEL C.; SAMPAIO, ALINE A.; ABDO, VICTORIA L.; NAGAY, BRUNA E.; CASTRO, NIDIA; RETAMAL-VALDES, BELEN; SHIBLI, JAMIL A.; FERES, MAGDA; BARAO, VALENTIM A. R.; et al. Cross-kingdom microbial interactions in dental implant-related infections: is Candida albicans a new villain?. ISCIENCE, v. 25, n. 4, p. 21-pg., . (20/05231-4, 20/10436-4, 19/17238-6)
SOUZA, JOAO GABRIEL S.; OLIVEIRA, BARBARA E. COSTA; COSTA, RAPHAEL C.; BECHARA, KAREN; CARDOSO-FILHO, OTAVIO; BENSO, BRUNA; SHIBLI, JAMIL AWAD; BERTOLINI, MARTINNA; BARAO, VALENTIM A. R.. Bacterial-derived extracellular polysaccharides reduce antimicrobial susceptibility on biotic and abiotic surfaces. ARCHIVES OF ORAL BIOLOGY, v. 142, p. 9-pg., . (20/05231-4, 20/10436-4)

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