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Microbial diversity in milk from healthy and mastitis cows from different regions of Brazil

Grant number: 19/17308-4
Support type:Regular Research Grants
Duration: December 01, 2020 - November 30, 2022
Field of knowledge:Agronomical Sciences - Veterinary Medicine
Principal researcher:Nathalia Cristina Cirone Silva
Grantee:Nathalia Cristina Cirone Silva
Home Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Assoc. researchers: André Thaler Neto ; Cintia Silva Minafra e Rezende ; Clarice Gebara Muraro Serrate Cordeiro Tenório ; Edmar Soares Nicolau ; Felipe de Freitas Guimarães ; Fernando Nogueira de Souza ; Helio Langoni ; Mônica Correia Gonçalves ; Mônica Maria Oliveira Pinho Cerqueira ; Rodrigo Carvalho Bicalho

Abstract

Mastitis in cattle is one of the diseases that most generates economic losses to the milk production chain due to reduced production, milk discard, premature slaughter, costs with veterinary treatment and alteration in milk quality. The study of microbial communities is in evidence and, currently, molecular methods have allowed a greater understanding of them. Staphylococcus spp. and Mycoplasma spp. are prevalent microorganisms as the cause of mastitis. Milk also has lactic bacteria that can be bacteriocin producers, having technological importance. The objective of this study is to verify the bacterial diversity of milk from healthy cows and with clinical and subclinical mastitis through the new generation sequencing of the 16S gene; isolate and characterize Staphylococcus sp. and lactic acid bacteria; to identify the presence of Mycoplasma sp. by PCR and to evaluate the relation of the microbiota of the raw milk with the quality of the milk in different regions of the country. Milk samples from healthy cows with subclinical and clinical mastitis will be collected from herds of dairy producing states from all regions of Brazil (South, Southeast, Midwest, North and Northeast). Milk samples will be evaluated for their composition (fat, protein, lactose, total solids, non-greasy solids, casein and urea nitrogen), Somatic Cell Count (CCS) and Total Bacterial Count (CBT) by electronic analysis. Total DNA from the milk will be extracted for PCR of Mycoplasma sp. identification and to carry out the new generation sequencing of the 16S rDNA gene for identification of the microbiota. In addition, lactic acid bacteria and Staphylococcus sp. will be isolated and characterized. For Staphylococcus sp. species, will be performed toxin production genes, biofilm and resistance profiles, as well as spa type and agr type will be identified. For lactic bacteria, the production capacity of bacteriocins will be evaluated as well their influence in the virulence expression of S. aureus. (AU)

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