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Multi-User Equipment approved in grant 2017/24453-5 Flow Cytometer

Grant number: 20/00665-6
Support Opportunities:Multi-user Equipment Program
Duration: April 01, 2020 - March 31, 2027
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal Investigator:Ana Paula Jacobus
Grantee:Ana Paula Jacobus
Host Institution: Instituto de Pesquisa em Bioenergia (IPBEN). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
Associated research grant:17/24453-5 - Collaborative network: modern genetic approaches to bust yeast tolerance to inhibitor-rich lignocellulosic hydrolysates, AP.BIOEN.JP
As informações de acesso ao Equipamento Multiusuário são de responsabilidade do Pesquisador responsável
EMU web page: Página do Equipamento Multiusuário não informada
Type of equipment:Processos Biológicos - Caracterização - Contagem células (inclui citômetros)
Manufacturer: Fabricante não informado
Model: Modelo não informado


The successful establishment of a second-generation (2G) ethanol industry requires key technological innovations that are still awaiting effective implementation. One of them is the development of yeast strains capable of withstanding toxic compounds (i.e., inhibitors) during fermentation of lignocellulosic hydrolysates (LCHs) derived from the sugarcane biomass. This project poses the question of how tolerance to LCH inhibitors can be improved in the yeast Saccharomyces cerevisiae by the use of modern molecular genetics and synthetic biology tools. To address this important issue, we established a collaborative axis between groups belonging to the recently launched Institute for Research in Bioenergy (IPBEN, UNESP), the Brazilian Bioethanol Science and Technology Laboratory (CTBE), and key partners from the University of São Paulo (USP) and the University of Queensland, Australia. The collaborative network will take advantage of innovative experimental approaches, such as alternative adaptive laboratory evolution protocols, quantitative trait loci mapping, next generation sequencing, and flow cytometry-assisted competition and phenotyping assays, to uncover the genetic basis of yeast tolerance to inhibitor-rich LCHs from the sugarcane bagasse. The produced knowledge will be resourceful for rationally designing a yeast strain hyper-tolerant to LCHs, which will be constructed by applying modern molecular genetics tools and the CRISPR/Cas9 genome editing technology. The resulting synthetic yeast is proposed to serve as a robust "chassis" upon which further genetic modifications (such as the metabolism of pentoses) might be added to yield a reference strain suited for cellulosic ethanol production. (AU)

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