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Effect of titanium dioxide nanotubes on biological properties of conventional glass ionomer cement

Abstract

Titanium dioxide nanotubes (n-TiO2) have been applied to dentistry to improve general properties of restorative materials. Our group has recently demonstrated that n-TiO2 may improve compression and wear resistance of conventional Glass Ionomer Cement (GIC), increase GIC hardness without affecting surface roughness, dentin micro shear-bond-strengh, and cell morphology as compared to GIC alone (Grant FAPESP #2016/13786-0). However, the ability of n-TiO2 to modulate bacteria and host response remains to be defined. Thus, the goals of the present study iare to: 1. define the impact of n-TiO2 on the antimicrobial properties of GIC; and 2. determine the potential of n-TiO2 to modulate the expression of immuno-inflammatory markers by fibroblasts (NIH/3T3 cells) treated or not with Fusobacterium nucleatum (Fn) total extract. In order to achieve those goals, n-TiO2 will be added at different concentrations (0%, 3%, 5%, 7% by weight) to GIC matrix (Ketac Molar EasyMix) and the following experiments performed: 1. Agar diffusion test (n=6): to determine a potential antimicrobial capacity of GIC, with or without, n-TiO2, bacteria inoculum (S. mutans or L. acidophilus) will be used, and chlorhexidine (0.12%) and distilled water will be used as positive and negative controls, respectively; 2. Cell viability (n=6): to assess the impact of GIC, with or without n-TiO2, on bacteria cell viability, 1x108 bacteria CFU/mL will be cultured in the presence of the tested materials and cell viability will be determined using the Live/Dead baclight fluorescence technique. Number of viable and non-viable cells will be obtained as the proportion of the total area of the disks in three random regions using the ImageJ program; 3. Cell morphology (n=6): the effect of tested materials on cell morphology will be defined by scanning electron microscopic analysis (SEM) (15 KV, 2000X), 4. Bacteria gene expression analysis (n=6): a potential impact of GIC, with or without n-TiO2, on transcript levels of vicR, covR, gtfB, gtfC, and egtfD in S. mutans and slpA, slpB e slpX for L. acidophilus will be determined by real-time quantitative PCR reactions; 5. Protein expression (n=6): NIH/3T3 cells will be cultured on GIC disks, with or without n-TiO2 (12 and 18 h), treated or not with Fn total extract (20, 2 and 0.2 ¼g/mL) and IL-1², IL-10, IL-6 and TNF secreted levels will be determined by mutiplex analysis using the MagPix system; and 6. Gene expression analysis in NIH/3T3 cells (n=6): IL-1², IL-10, IL-6, TNF transcript levels will be determined in NIH/3T3 cells cultured on GIC disks, with or without n-TiO2, treated or not with Fn total extract (3, 6 and 18 h). Experiments will be repeated, at least twice with three biological replicates, and in general, experimental times will be 1, 3 and 7 days (unless otherwise stated). Data were submitted to statistical analysis, considering the 5% level of significance (± = 0.05). (AU)

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VEICULO: TITULO (DATA)
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Publicações científicas (5)
(Referências obtidas automaticamente do Web of Science e do SciELO, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores)
ANA MARA DA SILVA MORAIS; YSADORA MAYARA REALI PEREIRA; ISAAC JORDÃO DE SOUZA-ARAÚJO; DANIEL FURTADO SILVA; VANESSA GALLEGO ARIAS PECORARI; ORISSON PONCE GOMES; FRANCISCO HUMBERTO NOCITI-JÚNIOR; REGINA MARIA PUPPIN-RONTANI; WALDEMIR FRANCISCO VIEIRA-JUNIOR; PAULO NORONHA LISBOA-FILHO; et al. TiO2 nanotube-containing glass ionomer cements display reduced aluminum release rates. Brazilian Oral Research, v. 36, . (19/14078-8, 16/13786-0)
DE SOUZA ARAUJO, ISAAC JORDAO; RICARDO, MARIANA GALLANTE; GOMES, ORISSON PONCE; GIOVANI, PRISCILA ALVES; PUPPIN-RONTANI, JULIA; PECORARI, VANESSA ARIAS; MARTINEZ, ELIZABETH FERREIRA; NAPIMOGA, MARCELO HENRIQUE; NOCITI JUNIOR, FRANCISCO HUMBERTO; PUPPIN-RONTANI, REGINA MARIA; et al. Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence. BRAZILIAN ORAL RESEARCH, v. 35, p. 12-pg., . (16/13786-0, 19/14078-8)
KANTOVITZ, KAMILA ROSAMILIA; CARLOS, NATALIA RUSSO; SILVA, IVANIA APARECIDA PIMENTA SANTOS; BRAIDO, CAROLINE; COSTA, BRUNA CAROLINA; KITAGAWA, IGOR LEBEDENCO; NOCITI-JR, FRANCISCO HUMBERTO; BASTING, ROBERTA TARKANY; DE FIGUEIREDO, FRANCINE KUHL PANZARELLA; LISBOA-FILHO, PAULO NORONHA. TiO2 nanotube-based nanotechnology applied to high-viscosity conventional glass-ionomer cement: ultrastructural analyses and physicochemical characterization. ODONTOLOGY, v. 111, n. 4, p. 13-pg., . (16/13786-0, 19/14078-8)
DA SILVA MORAIS, ANA MARA; REALI PEREIRA, YSADORA MAYARA; DE SOUZA-ARAUJO, ISAAC JORDAO; SILVA, DANIEL FURTADO; ARIAS PECORARI, VANESSA GALLEGO; GOMES, ORISSON PONCE; NOCITI-JUNIOR, FRANCISCO HUMBERTO; PUPPIN-RONTANI, REGINA MARIA; VIEIRA-JUNIOR, WALDEMIR FRANCISCO; LISBOA-FILHO, PAULO NORONHA; et al. TiO2 nanotube-containing glass ionomer cements display reduced aluminum release rates. BRAZILIAN ORAL RESEARCH, v. 36, p. 8-pg., . (16/13786-0, 19/14078-8)
ISAAC JORDÃO DE SOUZA ARAÚJO; MARIANA GALLANTE RICARDO; ORISSON PONCE GOMES; PRISCILA ALVES GIOVANI; JÚLIA PUPPIN-RONTANI; VANESSA ARIAS PECORARI; ELIZABETH FERREIRA MARTINEZ; MARCELO HENRIQUE NAPIMOGA; FRANCISCO HUMBERTO NOCITI JUNIOR; REGINA MARIA PUPPIN-RONTANI; et al. Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence. Brazilian Oral Research, v. 35, . (16/13786-0, 19/14078-8)

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