The use of molecular cytogenetics for evolutioonary and taxonomic studies in Cervidae

Abstract

The family Cervidae groups together species with a growing economic potential and endangered species. Karyotypes and phylogenetic position of many deer species are uncertain due to the lack of detailed scientific data. The extensive variation in chromosome numbers (2n=6-70) and structure makes this family suited for evolutionary studies based on interspecies molecular cytogenetic comparisons. In this project, we will perform a comparative analysis in a wide range of deer species, focusing on species lacking comprehensive data, including the genus Mazama and its cytotypes. We will determine karyotype differences and their impact on production of aneuploid spermatozoa and reproductive isolation, identify intrachromosomal rearrangements of the X chromosomes, and compare sequence similarities and chromosomal positions of satellite DNAs in various deer species. For this purpose, whole chromosome, BAC and satellite DNA probes for FISH will be constructed in VRI laboratory. Obtained data will be used to improve taxonomic classification, and review phylogenetic relationships within Cervidae. (AU)