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Bioprospecting of bacteriocin-producing bacteria: from culture optimization to the application in animal production systems

Grant number: 18/25511-1
Support type:Research Projects - Thematic Grants
Duration: November 01, 2019 - October 31, 2025
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal researcher:Ricardo Pinheiro de Souza Oliveira
Grantee:Ricardo Pinheiro de Souza Oliveira
Home Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Pesquisadores principais:
Alessandro de Mello Varani
Assoc. researchers:Alessandro de Mello Varani ; Attilio Converti ; Christian Hoffmann ; Danielle de Carla Dias ; Dirlei Antonio Berto ; Elias Gustavo Figueroa Villalobos ; Francisco Vitor Santos da Silva ; Iván Valdebenito Isler ; Jean Guy Joseph LeBlanc ; Jorge Gonzalo Farias Avendano ; José Manuel Domínguez González ; José Roberto Sartori ; Leonardo Tachibana ; Marcos Livio Panhoza Tse ; Maria Helena Ambrosio Zanin ; Maria José Tavares Ranzani de Paiva ; Patrícia Léo ; Paul Cotter ; Simone Maria Massami Kitamura Martins ; Welington Luiz de Araújo
Associated grant(s):21/15138-4 - Brewery waste biorefinery to obtain biologically active substances for prevention and treatment of SARS-CoV-2 and for its use as natural food preservatives, AV.EXT
20/15599-9 - Mult-user Equipment approved in grant 2018/25511-1: Semi-Preparative High Pressure Liquid Chromatography, AP.EMU
Associated scholarship(s):22/06012-0 - Antimicrobial potential of bacteriocins produced by lactic acid bacteria isolated from the intestinal tract of swine, BP.IC
22/04841-9 - Selection and identification of bacteriocin-producing probiotic strains, BP.PD
22/02936-2 - Probiotics and bacteriocins in Nile Tilapia feeding: effects on growth performance, immunology and experimental infection, BP.DD
+ associated scholarships 21/12583-7 - Genomic analysis of bacteriocinogenic probiotic bacteria: taxonomic identification and characterization of genes related to the synthesis of bacteriocins, BP.PD
21/01570-1 - Evaluating the potential of bacteriocinogenic lactic acid bacteria isolated from Rainbow Trout (Oncorhynchus mykiss): antimicrobial effect against Flavobacterium psychrophilum, BP.DR
20/14144-8 - Purification and encapsulation of bacteriocins from probiotic bacteria for applications in animal protein production systems, BP.PD
20/10676-5 - Selection of bacteriocinogenic probiotic strains, BP.PD
20/13271-6 - Production of bacteriocin-producing probiotic bacteria, BP.DD
20/03204-0 - Stability of PA-1 pediocin in the presence of choline-based osmolytes derived from phenolic compounds, BP.IC
20/03071-0 - Bioprospection of bacteriocinogenic probiotic bacteria from the intestinal tract of swine (Sus scrofa domesticus):antimicrobial effect against Escherichia coli, BP.IC - associated scholarships


The use of probiotic microorganisms in the prevention and treatment of bacterial infections in animals intended for human consumption has been considered an efficient alternative to the use of antibiotics. In addition, recent studies have shown that certain substances produced by these microorganisms, such as bacteriocins, vitamins, fatty acids, exopolysaccharides, enzymes, among others, improve the immunity and development of their hosts. The most commonly used probiotic microorganisms in the food and pharmaceutical industries are those belonging to the lactic acid bacteria group (LAB), since they are considered safe by regulatory agencies in this area. However, it is known that the beneficial effects generated by probiotics are specific to each host and, frequently, the compounds of interest are synthesized in greater quantities by a given bacterial strain. The main objective of the present study is the isolation and identification of probiotic LAB present in the intestinal microbiota of poultry, swine and fish species. Strains with high capacity to produce bacteriocins will be selected. From this selection, compatibility tests will be carried out among the strains and, later, a mix of probiotics will be prepared. The strains that compose it will be individually micro-encapsulated and administered daily in the animals diet, through feed and water, in order to ascertain the probiotic effectiveness of the mixture. Synthetic bacteriocins will also be micro-encapsulated and administered to understand their individual effect on animals health. The results obtained with microcapsules will be compared with those obtained in the same biosystems in free form. In addition, we will carry out immunological studies, electron microscopy tests and analysis of the intestinal microbiota diversity of animals. (AU)

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Scientific publications (10)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE SOUZA DE AZEVEDO, PAMELA OLIVEIRA; NOBREGA MENDONCA, CARLOS MIGUEL; RAMOS MORENO, ANA CAROLINA; IANK BUENO, ANTONIO VINICIUS; YOKOMIZO DE ALMEIDA, SONIA REGINA; SEIBERT, LIANE; CONVERTI, ATTILIO; WATANABE, II-SEI; GIERUS, MARTIN; DE SOUZA OLIVEIRA, RICARDO PINHEIRO. Antibacterial and antifungal activity of crude and freeze-dried bacteriocin-like inhibitory substance produced by Pediococcus pentosaceus. SCIENTIFIC REPORTS, v. 10, n. 1, . (18/25511-1, 18/04385-8)
MENDONCA, CARLOS M. N.; OLIVEIRA, RODRIGO C.; FREIRE, ROMINNE K. B.; PIAZENTIN, ANNA C. M.; PEREIRA, WELLISON A.; GUDINA, EDUARDO J.; EVTUGUIN, V, DMITRY; CONVERTI, ATTILIO; SANTOS, JOAO H. P. M.; NUNES, CLAUDIA; et al. Characterization of levan produced by a Paenibacillus sp. isolated from Brazilian crude oil. International Journal of Biological Macromolecules, v. 186, p. 788-799, . (20/13271-6, 18/25511-1)
PAZ, ALICIA; COSTA-TRIGO, IVAN; OLIVEIRA, RICARDO PINHEIRO DE SOUZA; DOMINGUEZ, JOSE MANUEL. Ligninolytic Enzymes of Endospore-Forming Bacillus aryabhattai BA03. Current Microbiology, v. 77, n. 5, p. 702-709, . (18/25511-1)
KUNIYOSHI, TAIS MAYUMI; NOBREGA MENDONCA, CARLOS MIGUEL; VIEIRA, VIVIANE BORGES; ROBL, DIOGO; GOMBOSSY DE MELO FRANCO, BERNADETTE DORA; TODOROV, SVETOSLAV DIMITROV; TOME, ELISABETTA; O'CONNOR, PAULA MARY; CONVERTI, ATTILIO; ARAUJO, WELINGTON LUIZ; et al. Pediocin PA-1 production by Pediococcus pentosaceus ET34 using non-detoxified hemicellulose hydrolysate obtained from hydrothermal pretreatment of sugarcane bagasse. APPLIED GEOCHEMISTRY, v. 131, . (18/25511-1, 15/24777-0, 13/07914-8, 18/04385-8)
COSTA-TRIGO, IVAN; PAZ, ALICIA; OTERO-PENEDO, PATRICIA; OUTEIRINO, DAVID; DE SOUZA OLIVEIRA, RICARDO PINHEIRO; MANUEL DOMINGUEZ, JOSE. Detoxification of chestnut burrs hydrolyzates to produce biomolecules. Biochemical Engineering Journal, v. 159, . (18/25511-1)
MEIRELES PIAZENTIN, ANNA CAROLINA; NOBREGA MENDONCA, CARLOS MIGUEL; VALLEJO, MARISOL; MUSSATTO, I, SOLANGE; DE SOUZA OLIVEIRA, RICARDO PINHEIRO. acteriocin-like inhibitory substances production by Enterococcus faecium 135 in co-culture with Ligilactobacillus salivarius and Limosilactobacillus reuter. Brazilian Journal of Microbiology, v. 53, n. 1, . (18/25511-1)
KUNIYOSHI, TAIS MAYUMI; NOBREGA MENDONCA, CARLOS MIGUEL; VIEIRA, VIVIANE BORGES; ROBL, DIOGO; GOMBOSSY DE MELO FRANCO, BERNADETTE DORA; TODOROV, SVETOSLAV DIMITROV; TOME, ELISABETTA; O'CONNOR, PAULA MARY; CONVERTI, ATTILIO; ARAUJO, WELINGTON LUIZ; et al. ediocin PA-1 production by Pediococcus pentosaceus ET34 using non-detoxified hemicellulose hydrolysate obtained from hydrothermal pretreatment of sugarcane bagass. Bioresource Technology, v. 338, . (13/07914-8, 18/04385-8, 15/24777-0, 18/25511-1)
VERISSIMO, NATHALIA VIEIRA; MUSSAGY, CASSAMO USSEMANE; OSHIRO, ARIANE ALVES; NOBREGA MENDONCA, CARLOS MIGUEL; SANTOS-EBINUMA, VALERIA DE CARVALHO; PESSOA JUNIOR, ADALBERTO; DE SOUZA OLIVEIRA, RICARDO PINHEIRO; BRANDAO PEREIRA, JORGE FERNANDO. From green to blue economy: Marine biorefineries for a sustainable ocean-based economy. GREEN CHEMISTRY, v. 23, n. 23, . (18/06908-8, 20/13271-6, 20/14144-8, 19/15493-9, 18/25511-1, 20/08655-0)
SABO, SABRINA DA SILVA; MENDES, MARIA ANITA; ARAUJO, ELIAS DA SILVA; DE ALMEIDA MURADIAN, LIGIA BICUDO; MAKIYAMA, EDSON NAOTO; GUY LEBLANC, JEAN; BORELLI, PRIMAVERA; FOCK, RICARDO AMBROSIO; KNOBL, TEREZINHA; DE SOUZA OLIVEIRA, RICARDO PINHEIRO. Bioprospecting of probiotics with antimicrobial activities against Salmonella Heidelberg and that produce B-complex vitamins as potential supplements in poultry nutrition. SCIENTIFIC REPORTS, v. 10, n. 1, . (18/25511-1, 17/16494-3)
DA SILVA, THAMIRES MARIA SIMOES; PIAZENTIN, ANNA CAROLINA MEIRELLES; MENDONCA, CARLOS MIGUEL NOBREGA; CONVERTI, ATTILIO; BOGSAN, CRISTINA STEWART BITTENCOURT; MORA, DIEGO; OLIVEIRA, RICARDO PINHEIRO DE SOUZA. Buffalo milk increases viability and resistance of probiotic bacteria in dairy beverages under in vitro simulated gastrointestinal conditions. JOURNAL OF DAIRY SCIENCE, v. 103, n. 9, p. 7890-7897, . (18/25511-1, 19/19054-0)

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