Comparative study between photodynamic therapy with urucum and LED and probiotics in halitosis reduction: controlled clinical trial

Abstract

Halitosis is a term that defines any odor or bad smell from the oral cavity, which may have local or systemic origin. This project aims to verify whether the treatment with antimicrobial photodynamic therapy (aPDT) and the treatment with probiotics are effective against it. Fifty-two students or UNINOVE employees, 18 to 25 years old, with a diagnosis of halitosis, will be selected, with sulphide (SH2) e 112 ppb in gas chromatography. Participants will be divided randomly into 4 groups of 13, who will receive different treatments: Group 1: treatment with brushing, dental floss and tongue scraper; Group 2: brushing, dental floss and aPTT applied to the back and middle third of the tongue; Group 3: brushing, dental floss and probiotics; Group 4: Brushing, flossing, aPDT and probiotics. The results of halimetry will be compared before, immediately after treatment, seven days after and thirty days after treatment. Microbiological analysis of the lingual flap will be performed at these same times. Quantitative analysis will be performed using real-time PCR. The normality of the data will be measured using the Shapiro-Wilk test, and in the case of normality the Variance Analysis (ANOVA) test will be applied, and in the case of non-parametric data, the Kruskal-Wallis test will be used. To analyze the results of each treatment in both periods of the study, the Wilcoxon test will be used. (AU)