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Investigation of the genomic methylation pattern in cattle related to parasite resistance

Abstract

Parasites are one of the main factors that can reduce productive and economic performance in cattle, and the most used way to control them is the use of chemicals. However, the indiscriminate use of antiparasitics may lead to the selection of resistant parasites and consequently the inefficacy of these drugs. Considering the importance of the control of parasites in cattle, it is necessary to search for alternatives such as the use of resistant animals and the study of the genetic diversity of the host genome. Thus, research on epigenetic mechanisms, such as DNA methylation, can be used to identify and possibly select resistant animals, since there are no studies in this area that correlate bovine DNA methylation patterns and parasitism. The objective of the present work is to characterize a herd of 72 ½ Angus x ½ Nellore heifers, identifying the resistant and susceptible animals to endo and ectoparasites, correlating the DNA methylation pattern of these animals and the degree of parasitism. The degree of individual parasitism of the animals (endo and ectoparasites) was evaluated by counting eggs per gram of feces, horn flies and ticks and the data will be submitted to ANOVA for later grouping by the Scott-Knott test at 5% of probability. The methylation pattern will be analyzed using the Imprint DNA Methylation Quantification Kit (Sigma)from the blood DNA extracted f blood by EasyPure Blood® Genomic DNA Kit (Transgen). Absorbance corrected values for each animal will be used for the analyzes of global methylation, which will be grouped into resistant, resilient and susceptible. The means of the methylation differences will be submitted to ANOVA by SISVAR software version 5.4, Tukey test analysis, to 95% reliability and the genetic distances will be calculated using the averages for later association of the animals of each group. (AU)

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VEICULO: TITULO (DATA)