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Effect of the exposure to fine particulate matter (PM2.5) on sperm functional quality of mice

Grant number: 19/05879-7
Support type:Regular Research Grants
Duration: December 01, 2019 - November 30, 2022
Field of knowledge:Health Sciences - Medicine
Principal researcher:Paula Intasqui Lopes
Grantee:Paula Intasqui Lopes
Home Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
Assoc. researchers:Mariana Matera Veras ; Ricardo Pimenta Bertolla

Abstract

Pollution, considered a growing public health problem, has been linked to reduced male fertility. Polluted air contains several toxic substances, of which we can highlight fine inhalable particulate matter (PM2.5), which promotes the disruption of the blood-testis barrier. Therefore, PM2.5 may reach the testis, and lead to testicular oxidative stress and DNA damage of germ cells. Thus, the objective of the present study is to evaluate the effect of PM2.5 on (i) chromatin protamination of testicular germ cells, (ii) sperm functional integrity, (iii) sperm DNA integrity, and (iii) reactive oxygen species production by epididymal sperm of mice. For this purpose, 30 male isogenic BALB/c mice with 21 days of age will be exposed to filtered air (control group, n=15) or to 600 ¼g PM2.5/m3 air (pollution group, n=15) until they reach 115 postnatal days. One day after the last exposure, animals will be sedated and sacrificed, and testis and epididymis will be collected. Testicular germ cells will be isolated for the expression analysis of genes related to chromatin protamination, using quantitative real time PCR, and analysis of global DNA methylation profile. Sperm collected from cauda epididymis will be used to evaluate functional integrity (mitochondrial activity and acrosome integrity), DNA integrity (DNA fragmentation levels and expression analysis of proteins related do chromatin protamination) and production of reactive oxygen species. With these results, we hope to obtain a better understanding of the mechanisms through which pollution may lead to male infertility. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
JEREMIAS, JESSICA TIMOTEO; BELARDIN, LARISSA BERLOFFA; OKADA, FATIMA KAZUE; ANTONIASSI, MARIANA P.; FRAIETTA, RENATO; BERTOLLA, RICARDO PIMENTA; INTASQUI, PAULA. Oxidative origin of sperm DNA fragmentation in the adult varicocele. INTERNATIONAL BRAZ J UROL, v. 47, n. 2, p. 275-283, . (14/17185-6, 14/11493-0, 19/05879-7)

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