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The Leishmania-host relationship from the ‘omics’ perspective

Abstract

Leishmania is the protozoan responsible for leishmaniasis, a neglected disease that in Brazil causes about 100,000 new cases per year. In its life cycle, the parasite is found in the midgut of an insect or inside the phagolysosome in the macrophage of the mammalian host. The different stages of parasite life cycle influence the regulation of gene expression, which occurs at post-transcriptional level. Applying genomic, transcriptomic and metabolomic approaches, we will continue, in four subprojects, to study the thematic established by our group, determining and/or establishing new correlations between arginine metabolism and polyamine production in the parasite-host interaction. Thus, we intend to characterize parasites resistant to pentamidine and duramycin, target drugs in polyamine pathways (subproject A). To determine how the infection changes the miRNA profile of human or mice macrophages correlating with transcripts and metabolites profiles (subproject B). To use CRISPR/Cas9 to obtain null knockouts parasites of genes involved in arginine metabolism, confirming physiological roles and the location of their products (subproject C). To correlate sleep fragmentation and consequent alteration of the mounting of immune response against Leishmania infection in C57BL/6 mice, elucidating if there are changes in the profile of transcripts and miRNAs that influence the immune response and the course of infection (subproject D). The challenge of the four sub-projects is to correlate the different aspects of the parasite-host interaction by "omic" analysis, raising points that can be explore the understanding of biology and physiology, and for the design of new strategies to combat the parasite. (AU)

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Scientific publications (11)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ACUNA, STEPHANIE MAIA; FLOETER-WINTER, LUCILE MARIA; MUXEL, SANDRA MARCIA. MicroRNAs: Biological Regulators in Pathogen-Host Interactions. CELLS, v. 9, n. 1, . (17/23519-2, 18/23512-0, 18/24693-9)
MARCIA MUXEL, SANDRA; MAMANI-HUANCA, MARICRUZ; AOKI, JULIANA IDE; ZAMPIERI, RICARDO ANDRADE; FLOETER-WINTER, LUCILE MARIA; LOPEZ-GONZALVEZ, ANGELES; BARBAS, CORAL. Metabolomic Profile of BALB/c Macrophages Infected with Leishmania amazonensis: Deciphering L-Arginine Metabolism. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 20, n. 24, . (17/23519-2, 18/23512-0, 18/24693-9, 16/03273-6)
SOUZA, MARINA DE ASSIS; RAMOS-SANCHEZ, EDUARDO MILTON; MUXEL, SANDRA MARCIA; LAGOS, DIMITRIS; REIS, LUIZA CAMPOS; PEREIRA, VALERIA REGO ALVES; BRITO, MARIA EDILEUZA FELINTO; ZAMPIERI, RICARDO ANDRADE; KAYE, PAUL MARTIN; FLOETER-WINTER, LUCILE MARIA; et al. miR-548d-3p Alters Parasite Growth and Inflammation in Leishmania (Viannia) braziliensis Infection. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 11, . (18/23512-0, 14/14756-2, 19/25393-1, 18/14398-0, 18/24693-9)
RAMOS-SANCHEZ, EDUARDO MILTON; REIS, LUIZA CAMPOS; SOUZA, MARINA DE ASSIS; MUXEL, SANDRA MARCIA; SANTOS, KAMILA REIS; LAGOS, DIMITRIS; PEREIRA, VALERIA REGO ALVES; DE BRITO, MARIA EDILEUZA FELINTO; KAYE, PAUL MARTIN; FLOETER-WINTER, LUCILE MARIA; et al. miR-548d-3p Is Up-Regulated in Human Visceral Leishmaniasis and Suppresses Parasite Growth in Macrophages. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 12, p. 14-pg., . (19/25393-1, 14/14756-2, 18/14398-0, 18/24693-9, 18/23512-0)
AOKI, JULIANA IDE; MUXEL, SANDRA MARCIA; ZAMPIERI, RICARDO ANDRADE; MUELLER, KARL ERIK; NERLAND, AUDUN HELGE; FLOETER-WINTER, LUCILE MARIA. Differential immune response modulation in early Leishmania amazonensis infection of BALB/c and C57BL/6 macrophages based on transcriptome profiles (vol 9, 19841, 2019). SCIENTIFIC REPORTS, v. 10, n. 1, p. 1-pg., . (18/24693-9, 14/50717-1, 16/03273-6, 18/23512-0)
AOKI, JULIANA IDE; MUXEL, SANDRA MARCIA; LARANJEIRA-SILVA, MARIA FERNANDA; ZAMPIERI, RICARDO ANDRADE; MULLER, KARL ERIK; NERLAND, AUDUN HELGE; FLOETER-WINTER, LUCILE MARIA. Dual transcriptome analysis reveals differential gene expression modulation influenced by Leishmania arginase and host genetic background. MICROBIAL GENOMICS, v. 6, n. 9, p. 13-pg., . (14/50717-1, 16/03273-6, 17/23933-3, 18/24693-9, 18/23512-0)
HONG, AHYUN; ZAMPIERI, RICARDO ANDRADE; SHAW, JEFFREY JON; FLOETER-WINTER, LUCILE MARIA; LARANJEIRA-SILVA, MARIA FERNANDA. One Health Approach to Leishmaniases: Understanding the Disease Dynamics through Diagnostic Tools. PATHOGENS, v. 9, n. 10, . (17/23933-3, 18/23512-0)
CORTAZZO DA SILVA, LEONARDO; AOKI, JULIANA IDE; FLOETER-WINTER, LUCILE MARIA. Finding Correlations Between mRNA and Protein Levels in Leishmania Development: Is There a Discrepancy?. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 12, p. 16-pg., . (17/23696-1, 18/23512-0, 21/04422-3)
MAMANI-HUANCA, MARICRUZ; MUXEL, SANDRA MARCIA; ACUNA, STEPHANIE MAIA; FLOETER-WINTER, LUCILE MARIA; BARBAS, CORAL; LOPEZ-GONZALVEZ, ANGELES. Metabolomic Reprogramming of C57BL/6-Macrophages during Early Infection with L. amazonensis. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 22, n. 13, . (17/23519-2, 18/24693-9, 18/23512-0)
BOY, ROMARIO LOPES; HONG, AHYUN; AOKI, JULIANA IDE; FLOETER-WINTER, LUCILE MARIA; LARANJEIRA-SILVA, MARIA FERNANDA. Reporter gene systems: A powerful tool for Leishmania studies. CURRENT RESEARCH IN MICROBIAL SCIENCES, v. 3, p. 11-pg., . (17/23933-3, 18/23512-0)
ZAMPIERI, RICARDO ANDRADE; AOKI, JULIANA IDE; MUELLER, KARL ERIK; SHAW, JEFFREY JON; FLOETER-WINTER, LUCILE MARIA. Comparison of Sampling Procedures for the Molecular Diagnosis of Leishmaniases. American Journal of Tropical Medicine and Hygiene, v. 108, n. 3, p. 7-pg., . (14/50717-1, 18/23512-0)

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