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Enzymatic biotechnology for the xylo-oligosaccharides production from lignocellulosic agroindustrial residues aimed the production of symbiotics

Grant number: 19/08542-3
Support Opportunities:Regular Research Grants
Duration: October 01, 2019 - July 31, 2022
Field of knowledge:Agronomical Sciences - Food Science and Technology - Food Engineering
Principal Investigator:Rosana Goldbeck Coelho
Grantee:Rosana Goldbeck Coelho
Host Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated researchers:Ana Carla Kawazoe Sato


Lignocellulosic materials represent a source of under-exploited raw material in biotechnological processes, since a large surplus of waste from the industrial processing of agricultural raw materials is generated annually. Thus, the production of xylo-oligosaccharides (XOs) from the enzymatic hydrolysis of xylan present in the hemicellulose from these residues presents a smart and sustainable alternative for their valorization, as well as the production of industrial inputs with high added value . Xylo-oligosaccharides (XOs) are distinguished by their prebiotic activities that favor an improvement in intestinal functions, immunological action, antimicrobial and other health benefits. In this sense, the present proposal aims to produce xylo-oligosaccharides from the enzymatic hydrolysis of agroindustrial lignocellulosic residues, aiming at the association with probiotic bacteria to obtain symbiotic microcapsules. In this work two agroindustrial residues from state of São Paulo will be tested: sugar cane straw and coffee husk. In a first stage the agroindustrial residues will be characterized as to the content of xylan present. Afterwards, experimental design will be carried out in order to select the enzymes that in fact have a significant impact on the release of XOs with polymerization degree (GP) of 2 to 6, as well as to optimize optimal concentrations of enzymes for the production of XOs. In parallel, a study will be carried out on the symbiotic association of XOs and probiotic bacteria, in order to obtain symbiotic microcapsules. By means of in vitro assays, the digestibility of the microcapsules obtained will be analyzed, which will be evaluated the resistance to the hydrolysis of enzymes of the digestive tract, as well as the stimulation of the growth of the probiotic bacteria. Finally, it is expected at the end of the project that the microcapsules produced from the xylo-oligosaccharides from lignocellulosic agroindustrial residues present symbiotic potential so that they can be applied industrially as functional ingredients in food products. It is important to mention that this line of research was initiated in the post-doctoral study of the researcher in question (responsible for the proposal) that was funded by FAPESP (12 / 18859-5) and is currently strengthening this line of research at School of Food Engineering - UNICAMP) where she is professor. (AU)

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Scientific publications (28)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SANTOS, BRUNO A. S.; AZAMBUJA, SUELLEN P. H.; AVILA, PATRICIA F.; PACHECO, MARIA TERESA B.; GOLDBECK, ROSANA. n-Butanol production bySaccharomyces cerevisiaefrom protein-rich agro-industrial by-products. Brazilian Journal of Microbiology, v. 51, n. 4, . (19/08542-3, 16/04602-3, 15/50612-8, 15/20630-4)
AZAMBUJA, SUELLEN P. H.; GOLDBECK, ROSANA. Butanol production by Saccharomyces cerevisiae: perspectives, strategies and challenges. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, v. 36, n. 3, . (19/08542-3, 15/20630-4)
MONTEIRO, CARLA R. M.; AVILA, PATRICIA F.; PEREIRA, MARIA ANGELICA F.; PEREIRA, GABRIELA N.; BORDIGNON, SIDNEI E.; ZANELLA, EDUARDO; STAMBUK, BORIS U.; DE OLIVEIRA, DEBORA; GOLDBECK, ROSANA; POLETTO, PATRICIA. Hydrothermal treatment on depolymerization of hemicellulose of mango seed shell for the production of xylooligosaccharides. Carbohydrate Polymers, v. 253, . (19/08542-3)
BARBOSA, FERNANDO CESAR; KENDRICK, EMANUELE; BRENELLI, LIVIA BEATRIZ; ARRUDA, HENRIQUE SILVANO; PASTORE, GLAUCIA MARIA; RABELO, SARITA CANDIDA; DAMASIO, ANDRE; FRANCO, TELMA TEIXEIRA; LEAK, DAVID; GOLDBECK, ROSANA. Optimization of cello-oligosaccharides production by enzymatic hydrolysis of hydrothermally pretreated sugarcane straw using cellulolytic and oxidative enzymes. BIOMASS & BIOENERGY, v. 141, . (17/16159-0, 17/22669-0, 15/50612-8, 19/08542-3)
MARTINS, MANOELA; SGANZERLA, WILLIAM GUSTAVO; FORSTER-CARNEIRO, TANIA; GOLDBECK, ROSANA. Recent advances in xylo-oligosaccharides production and applications: A comprehensive review and bibliometric analysis. BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, v. 47, p. 16-pg., . (19/26925-7, 19/08542-3, 18/14938-4, 20/02871-2)
MARTINS, MANOELA; SILVA, MARCOS FELLIPE; DINAMARCO, TAISA MAGNANI; GOLDBECK, ROSANA. Novel bi-functional thermostable chimeric enzyme for feasible xylo-oligosaccharides production from agro-industrial wastes. Process Biochemistry, v. 122, p. 10-pg., . (20/02871-2, 19/08542-3)
SILVELLO, MARIA AUGUSTA DE CARVALHO; GASPAROTTO, GIOVANNA ALVES; FERREIRA, GABRIELA F.; SANTOS, LUCIELEN OLIVEIRA; FREGOLENTE, LEONARDO V.; GOLDBECK, ROSANA. Nutrient Optimization Strategy to Increase the Carbohydrate Content of Chlorella vulgaris and Evaluation of Hydrolysis and Fermentation Performance. BioEnergy Research, v. N/A, p. 10-pg., . (19/08542-3, 18/20787-9, 15/20630-4)
MARTINS, MANOELA; TRAMONTINA, ROBSON; SQUINA, FABIO MARCIO; DINAMARCO, TAISA MAGNANI; GOLDBECK, ROSANA. Synergism for xylo-oligosaccharides, p-coumaric and ferulic acid production, and thermostability modulation of GH 62 alpha-L-arabinofuranosidase. BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, v. 44, p. 11-pg., . (20/02871-2, 15/50590-4, 19/08542-3)
SGANZERLA, WILLIAM GUSTAVO; DA SILVA, MARCOS FELLIPE; ZABOT, GIOVANI L.; GOLDBECK, ROSANA; MUSSATTO, SOLANGE I.; FORSTER-CARNEIRO, TANIA. Techno-economic assessment of subcritical water hydrolysis of brewer?s spent grains to recover xylo-oligosaccharides. JOURNAL OF SUPERCRITICAL FLUIDS, v. 196, p. 12-pg., . (19/26925-7, 19/08542-3, 18/14938-4, 21/12762-9)
DA SILVA, AURENICE MARIA MOTA; ALMEIDA, FLAVIA SOUZA; DA SILVA, MARCOS FELLIPE; GOLDBECK, ROSANA; SATO, ANA CARLA KAWAZOE. How do pH and temperature influence extraction yield, physicochemical, functional, and rheological characteristics of brewer spent grain protein concentrates?. FOOD AND BIOPRODUCTS PROCESSING, v. 139, p. 12-pg., . (19/08542-3, 18/21987-1, 11/06083-0, 09/54137-1, 15/11984-7, 19/27354-3)
AVILA, PATRICIA F.; DE MELLO, ALLAN H. F.; FORTE, MARCUS B. S.; COSTA, FATIMA A. DE ALMEIDA; GOLDBECK, ROSANA. Fractionation of functional oligosaccharides produced from sugarcane straw using serial nanofiltration membranes and their influence on prebiotic potential. Food Research International, v. 172, p. 7-pg., . (19/08542-3, 17/24503-2)
AVILA, PATRICIA F.; DE MELLO, ALLAN H. F.; GOLDBECK, ROSANA. Cello-oligosaccharides production from multi-stage enzymatic hydrolysis by lignocellulosic biomass and evaluation of prebiotic potential. INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES, v. 85, p. 8-pg., . (19/08542-3, 17/24503-2)
OLIVEIRA, T. C. G.; INTERLANDI, M. A.; HANLON, K. E.; TORRES-MAYANGA, P. C.; SILVELLO, M. A. C.; TIMKO, M. T.; ROSTAGNO, M. A.; GOLDBECK, R.; FORSTER-CARNEIRO, T.. Integration of Subcritical Water and Enzymatic Hydrolysis to Obtain Fermentable Sugars and Second-Generation Ethanol from Sugarcane Straw. BioEnergy Research, v. 15, n. 2, p. 12-pg., . (19/08542-3, 18/05999-0, 18/14582-5, 13/04304-4, 18/14938-4)
OLIVEIRA, T. C. G.; HANLON, K. E.; INTERLANDI, M. A.; TORRES-MAYANGA, P. C.; SILVELLO, M. A. C.; LACHOS-PEREZ, D.; TIMKO, M. T.; ROSTAGNO, M. A.; GOLDBECK, R.; FORSTER-CARNEIRO, T.. Subcritical water hydrolysis pretreatment of sugarcane bagasse to produce second generation ethanol. JOURNAL OF SUPERCRITICAL FLUIDS, v. 164, . (18/14582-5, 18/23835-4, 18/14938-4, 18/05999-0, 19/08542-3)
BARBOSA, FERNANDO CESAR; SILVELLO, MARIA AUGUSTA; GOLDBECK, ROSANA. Cellulase and oxidative enzymes: new approaches, challenges and perspectives on cellulose degradation for bioethanol production. Biotechnology Letters, v. 42, n. 6, p. 875-884, . (18/20787-9, 19/08542-3, 17/16159-0)
LOPES, ALBERTO MOURA MENDES; MARTINS, MANOELA; GOLDBECK, ROSANA. Heterologous Expression of Lignocellulose-Modifying Enzymes in Microorganisms: Current Status. MOLECULAR BIOTECHNOLOGY, v. 63, n. 3, p. 184-199, . (19/08542-3)
MARTINS, MANOELA; DINAMARCO, TAISA MAGNANI; GOLDBECK, ROSANA. Recombinant chimeric enzymes for lignocellulosic biomass hydrolysis. Enzyme and Microbial Technology, v. 140, . (19/08542-3)
AVILA, PATRICIA F.; MARTINS, MANOELA; GOLDBECK, ROSANA. Enzymatic Production of Xylooligosaccharides from Alkali-Solubilized Arabinoxylan from Sugarcane Straw and Coffee Husk. BioEnergy Research, v. 14, n. 3, p. 739-751, . (17/24503-2, 19/08542-3, 15/50612-8, 15/20630-4)
MARTINS, MANOELA; SATO, ANA CARLA KAWAZOE; OGINO, KENJI; GOLDBECK, ROSANA. valuating the addition of xylooligosaccharides into alginate-gelatin hydrogel. Food Research International, v. 147, . (18/14223-5, 19/08542-3, 15/20630-4)
SILVELLO, MARIA AUGUSTA DE CARVALHO; CAMARGO, ALINE FRUMI; SCAPINI, THAMARYS; PAUDEL, SHUKRA RAJ; TREICHEL, HELEN; GOLDBECK, ROSANA. Enzymatic Hydrolysis Intensification of Lignocellulolytic Enzymes Through Ultrasonic Treatment. BioEnergy Research, . (19/08542-3, 15/20630-4, 18/20787-9)
PEREIRA, MARIA ANGELICA F.; MONTEIRO, CARLA ROANA M.; PEREIRA, GABRIELA N.; JUNIOR, SIDNEI E. BORDIGNON; ZANELLA, EDUARDO; AVILA, PATRICIA F.; STAMBUK, BORIS U.; GOLDBECK, ROSANA; DE OLIVEIRA, DEBORA; POLETTO, PATRICIA. Deconstruction of banana peel for carbohydrate fractionation. Bioprocess and Biosystems Engineering, v. 44, n. 2, p. 297-306, . (19/08542-3)
AVILA, PATRICIA F.; SILVA, MARCOS F.; MARTINS, MANOELA; GOLDBECK, ROSANA. Cello-oligosaccharides production from lignocellulosic biomass and their emerging prebiotic applications. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, v. 37, n. 5, . (19/08542-3, 15/20630-4, 15/50612-8, 17/24503-2)
SAMPAIO, ULLIANA MARQUES; DA SILVA, MARCOS FELLIPE; GOLDBECK, ROSANA; CLERICI, MARIA TERESA PEDROSA SILVA. Technological and prebiotic aspects of young bamboo culm flour (Dendrocalamus latiflorus) combined with rice flour to produce healthy extruded products. Food Research International, v. 165, p. 12-pg., . (19/08542-3)
MACIEL-SILVA, FRANCISCO W.; LACHOS-PEREZ, DANIEL; BULLER, LUZ SELENE; SGANZERLA, WILLIAM G.; PEREZ, MONTSERRAT; ROSTAGNO, MAURICIO A.; FORSTER-CARNEIRO, TANIA. Green Extraction Processes for Complex Samples from Vegetable Matrices Coupled with On-Line Detection System: A Critical Review. Molecules, v. 27, n. 19, p. 21-pg., . (18/14582-5, 20/10323-5, 19/08542-3, 18/14938-4)
MARTINS, MANOELA; AVILA, PATRICIA FELIX; PAIM DE ANDRADE, CRISTIANE CONTE; GOLDBECK, ROSANA. Synergic recombinant enzyme association to optimize xylo-oligosaccharides production from agricultural waste. BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, v. 28, p. 8-pg., . (15/20630-4, 18/14223-5, 15/50612-8, 19/08542-3)
MORETTI, LETICIA KORIN; RAMOS, KAZUMI KAWASAKI; AVILA, PATRICIA FELIX; GOLDBECK, ROSANA; VIEIRA, JOSELIO BATISTA; EFRAIM, PRISCILLA. Influence of cocoa varieties on carbohydrate composition and enzymatic activity of cocoa pulp. Food Research International, v. 173, p. 8-pg., . (19/08542-3)
BARBOSA, FERNANDO CESAR; MARTINS, MANOELA; BRENELLI, LIVIA BEATRIZ; FERRARI, FELIPE AUGUSTO; SOARES FORTE, MARCUS BRUNO; RABELO, SARITA CANDIDA; FRANCO, TELMA TEIXEIRA; GOLDBECK, ROSANA. Screening of potential endoglucanases, hydrolysis conditions and different sugarcane straws pretreatments for cello-oligosaccharides production. Bioresource Technology, v. 316, p. 8-pg., . (18/14223-5, 19/08542-3, 17/15477-8, 15/50612-8, 19/19976-4)

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