Microfluidics platform to obtain genetic information and predict genetic profile of individual patients for medical doctors and hospitals, based on a fast, integrated DNA treatment, sequencing, and data analysis solution in personalized medicine

Abstract

This project aims to develop a microfluidic platform able to perform genetic tests of patients for medical doctors. For this, an automated and miniaturized device named 2-seq has been created, so as the doctors can insert a biological sample of their patients into the equipment, while being in their office. By performing experimental protocols in locu, the device starts from processing samples containing DNA, then automatically generates genetic test as final results. This is only possible, because this tailored device is able to put together, in a miniaturized and coupled fashion, experimental operations needed to run molecular protocols, such as electrowetting display, mixing, vortex, electromagnetics, PCR, spectrophotometer, and DNA sequencing. What makes this solution unique is the use of a state-of-the-art technology, controlled by a microcontroller and a firmware, which connects these devices through a system of electrowetting, microfluidics, and electronic circuits. Thus, by using the 2-seq platform, experimental protocols previously performed separately and exhaustively in laboratories by specialized technicians, can now be quickly carried out without human intervention. Finally, the device 2-seq also provides users with a software for them to choose which operation and genetic tests they wish to get results from, in a simple and intuitive way. (AU)