Advanced search
Start date
Betweenand
Related content

Study of different antigenic preparations of Paracoccidioides lutzii for the standardization of the ELISA test as an aid in the diagnosis of paracoccidioidomycosis due to P. lutzii

Grant number: 18/21460-3
Support Opportunities:Regular Research Grants
Duration: August 01, 2019 - July 31, 2022
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal Investigator:Zoilo Pires de Camargo
Grantee:Zoilo Pires de Camargo
Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
Associated researchers:Anderson Messias Rodrigues ; Eva Burger ; Olga Fischman Gompertz ; Rosane Christine Hahn

Abstract

Traditionally, paracoccidioidomycosis (PCM) is known to be a deep mycosis caused by the thermodymorphic fungus Paracoccidioides brasiliensis. However, there are indications of other species of Paracoccidioides. One such species is P. lutzii, previously named Pb01-like. The other species are P. brasiliensis S1, PS2, PS3 and PS4, recently named P. brasiliensis sensu stricto, P. americana, P. restrepiensis and P. venezuelensis, respectively. The disease is limited to Latin America countries and very little is known about their ecologies. The highest incidence of P. lutzii is in the center-west region of Brazil; however, it may occur in other regions as well. The diversity of clinical forms of the disease is related to the host's immune status and intrinsic characteristics of the fungus. Antigenic proteins may be used as serological markers in the development of more specific diagnostic tests. Conventional serological tests, such as immunodiffusion, using P. brasiliensis exoantigen cannot diagnose PCM due P. lutzii. In this project, we intend to standardize the ELISA test to aid in the diagnosis of PCM by P. lutzii. For this purpose, several types of antigenic preparations derived from P. lutzii will be tested using sera from PCM patients due to P. lutzii and P. brasiliensis. (AU)

Articles published in Agência FAPESP Newsletter about the research grant:
More itemsLess items
Articles published in other media outlets ( ):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Scientific publications (18)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE CARVALHO, JAMILE AMBROSIO; HAGEN, FERRY; FISHER, MATTHEW C.; DE CAMARGO, ZOILO PIRES; RODRIGUES, ANDERSON MESSIAS. Genome-wide mapping using new AFLP markers to explore intraspecific variation among pathogenicSporothrixspecies. PLoS Neglected Tropical Diseases, v. 14, n. 7, . (18/21460-3, 17/27265-5)
LOSADA, LUIZA CHAVES DE MIRANDA LEONHARDT; MONTEIRO, RUAN CAMPOS; DE CARVALHO, JAMILE AMBROSIO; HAGEN, FERRY; FISHER, MATTHEW C.; SPRUIJTENBURG, BRAM; MEIS, JACQUES F.; DE GROOT, THEUN; GONCALVES, SARAH SANTOS; NEGRONI, RICARDO; et al. High-Throughput Microsatellite Markers Development for Genetic Characterization of Emerging Sporothrix Species. JOURNAL OF FUNGI, v. 9, n. 3, p. 34-pg., . (18/21460-3, 17/27265-5)
DE CARVALHO, JAMILE AMBROSIO; HAGEN, FERRY; FISHER, MATTHEW C.; DE CAMARGO, ZOILO PIRES; RODRIGUES, ANDERSON MESSIAS. Genome-wide mapping using new AFLP markers to explore intraspecific variation among pathogenic Sporothrix species. PLoS Neglected Tropical Diseases, v. 14, n. 7, p. 26-pg., . (18/21460-3, 17/27265-5)
KRUSCHEWSKY, WDSON LUIS LIMA; PATRICIO, SUZANA ALVES; BAHIENSE, ISABELA CRUZ; MAIFREDE, SIMONE BRAVIM; POSSA, ANA PAULA; DE CAMARGO, ZOILO PIRES; RODRIGUES, ANDERSON MESSIAS; PECANHA, PAULO MENDES; FALQUETO, ALOISIO; GRAO-VELLOSO, TANIA REGINA; et al. Paracoccidioidomycosis cases by Paracoccidioides lutzii in southeastern Brazil. Brazilian Journal of Microbiology, v. N/A, p. 7-pg., . (18/21460-3, 17/27265-5)
RODRIGUES, ANDERSON MESSIAS; DELLA TERRA, PAULA PORTELLA; GREMIAO, ISABELLA DIB; PEREIRA, SANDRO ANTONIO; OROFINO-COSTA, ROSANE; DE CAMARGO, ZOILO PIRES. The threat of emerging and re-emerging pathogenic Sporothrix species. Mycopathologia, v. 185, n. 5, p. 30-pg., . (18/21460-3, 17/27265-5)
NERY, ANDREIA FERREIRA; DE CAMARGO, ZOILO PIRES; RODRIGUES, ANDERSON MESSIAS; PORTELA, TIAGO FERREIRA; HOFFMANN-SANTOS, HUGO DIAS; PINHEIRO, BRENO GONCALVES; POSSA, ANA PAULA; DA SILVA CAVALCANTE, LETICIA ROSSETTO; HAGEN, FERRY; HAHN, ROSANE CHRISTINE. Puzzling paracoccidioidomycosis: Factors associated with the severity of Paracoccidioides lutzii infections. INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, v. 107, p. 284-290, . (17/27265-5, 18/21460-3)
NERY, ANDREIA FERREIRA; DE CAMARGO, ZOILO PIRES; RODRIGUES, ANDERSON MESSIAS; PORTELA, TIAGO FERREIRA; HOFFMANN-SANTOS, HUGO DIAS; KRUGER DAMBROS, PEDRO VITOR; RIBEIRO DE SOUZA, JANIO FELIPE; GARCIA, ALEXANDRE CARVALHO; DAMASIO SANTOS, CAROLINA ARAUJO; HAGEN, FERRY; et al. Paracoccidioidomycosis due to P lutzii: The importance of neutrophil/lymphocyte ratio in the symptomatic and asymptomatic phases in severe cases. MYCOSES, v. 64, n. 8, p. 874-881, . (17/27265-5, 18/21460-3)
RODRIGUES, ANDERSON MESSIAS; KUBITSCHEK-BARREIRA, PAULA HELENA; PINHEIRO, BRENO GONCALVES; TEIXEIRA-FERREIRA, ANDRE; HAHN, ROSANE CHRISTINE; DE CAMARGO, ZOILO PIRES. Immunoproteomic Analysis Reveals Novel Candidate Antigens for the Diagnosis of Paracoccidioidomycosis Due to Paracoccidioides lutzii. JOURNAL OF FUNGI, v. 6, n. 4, . (18/21460-3, 17/27265-5)
DE CARVALHO, J. A.; BEALE, M. A.; HAGEN, F.; FISHER, M. C.; KANO, R.; BONIFAZ, A.; TORIELLO, C.; NEGRONI, R.; REGO, R. S. DE M.; GREMIAO, I. D. F.; et al. rends in the molecular epidemiology and population genetics of emerging Sporothrix specie. STUDIES IN MYCOLOGY, n. 100, . (17/27265-5, 18/21460-3)
RODRIGUES, A. M.; BEALE, M. A.; HAGEN, F.; FISHER, M. C.; TERRA, P. P. D.; DE HOOG, S.; BRILHANTE, R. S. N.; DE AGUIAR CORDEIRO, R.; DE SOUZA COLLARES MAIA CASTELO-BRANCO, D.; ROCHA, M. F. G.; et al. The global epidemiology of emerging Histoplasma species in recent years. STUDIES IN MYCOLOGY, n. 97, . (17/27265-5, 18/21460-3)
RODRIGUES, ANDERSON MESSIAS; DELLA TERRA, PAULA PORTELLA; GREMIAO, ISABELLA DIB; PEREIRA, SANDRO ANTONIO; OROFINO-COSTA, ROSANE; DE CAMARGO, ZOILO PIRES. The threat of emerging and re-emerging pathogenic Sporothrix species. Mycopathologia, v. 185, n. 5, SI, . (17/27265-5, 18/21460-3)
DELLA TERRA, PAULA PORTELLA; GONSALES, FERNANDA FIDELIS; DE CARVALHO, JAMILE AMBROSIO; HAGEN, FERRY; KANO, RUI; BONIFAZ, ALEXANDRO; DE CAMARGO, ZOILO PIRES; RODRIGUES, ANDERSON MESSIAS. Development and evaluation of a multiplex qPCR assay for rapid diagnostics of emerging sporotrichosis. TRANSBOUNDARY AND EMERGING DISEASES, . (18/21460-3, 17/27265-5)
ROBERTO, T. N.; DE CARVALHO, J. A.; BEALE, M. A.; HAGEN, F.; FISHER, M. C.; HAHN, R. C.; DE CAMARGO, Z. P.; RODRIGUES, A. M.. xploring genetic diversity, population structure, and phylogeography in Paracoccidioides species using AFLP marker. STUDIES IN MYCOLOGY, n. 100, . (13/05405-9, 17/27265-5, 18/21460-3)
HAHN, ROSANE CHRISTINE; HAGEN, FERRY; MENDES, RINALDO PONCIO; BURGER, EVA; NERY, ANDREIA FERREIRA; SIQUEIRA, NATHAN PEREIRA; GUEVARA, ARMANDO; RODRIGUES, ANDERSON MESSIAS; DE CAMARGO, ZOILO PIRES. Paracoccidioidomycosis: Current Status and Future Trends. CLINICAL MICROBIOLOGY REVIEWS, v. 35, n. 4, p. 65-pg., . (17/27265-5, 18/21460-3)
PINHEIRO, BRENO GONCALVES; HAHN, ROSANE CHRISTINE; DE CAMARGO, ZOILO PIRES; RODRIGUES, ANDERSON MESSIAS. Molecular Tools for Detection and Identification of Paracoccidioides Species: Current Status and Future Perspectives. JOURNAL OF FUNGI, v. 6, n. 4, . (17/27265-5, 18/21460-3)
RODRIGUES, A. M.; BEALE, M. A.; HAGEN, F.; FISHER, M. C.; TERRA, P. P. D.; DE HOOG, S.; BRILHANTE, R. S. N.; DE AGUIAR CORDEIRO, R.; DE SOUZA COLLARES MAIA CASTELO-BRANCO, D.; ROCHA, M. F. G.; et al. The global epidemiology of emerging Histoplasma species in recent years. STUDIES IN MYCOLOGY, v. N/A, n. 97, p. 21-pg., . (18/21460-3, 17/27265-5)
MAIFREDE, SIMONE BRAVIM; KRUSCHEWSKY, WDSON LUIS LIMA; PATRICIO, SUZANA ALVES; FALQUETO, ALOISIO; PECANHA, PAULO MENDES; MALAQUIAS, LUIZ COSME COTTA; POSSA, ANA PAULA; DE CAMARGO, ZOILO PIRES; RODRIGUES, ANDERSON MESSIAS; GONCALVES, SARAH SANTOS; et al. Screening paracoccidioidomycosis by double immunodiffusion test in a referred diagnostic center in Brazilian southeastern: an accessible tool. INFECTION, v. 49, n. 6, . (18/21460-3, 17/27265-5)
PINHEIRO, BRENO GONCALVES; POSSA, ANA PAULA; DELLA TERRA, PAULA PORTELLA; DE CARVALHO, JAMILE AMBROSIO; RICCI, GIANNINA; NISHIKAKU, ANGELA SATIE; HAHN, ROSANE CHRISTINE; CAMARGO, ZOILO PIRES DE; RODRIGUES, ANDERSON MESSIAS. A New Duplex PCR-Assay for the Detection and Identification of Paracoccidioides Species. JOURNAL OF FUNGI, v. 7, n. 3, . (17/27265-5, 18/21460-3)

Please report errors in scientific publications list using this form.
X

Report errors in this page


Error details: