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Proteomic profile of bacteria from root canals of symptomatic or asymptomatic teeth with apical periodontitis


Endodontic infections are caused by a multi-species community of bacteria. It is difficult to infer the physiology, function and microbial pathogenicity with methods of microbiological identification and quantification of endotoxins. Thus, proteomic analysis is a technique that can revolutionize the study of the pathogenesis of endodontic infections. The present study aims to: analyze the proteomic profile of bacteria present in root canals of teeth with symptomatic and asymptomatic apical periodontitis; to broaden the understanding of the role of the proteome in the pathogenesis of periapical diseases; to investigate the correlation of the composition of the bacterial proteins detected with the presence of clinical signs and symptoms. Samples will be collected from 18 patients referred for root canal treatment or emergency treatment at the Araçatuba Dental School FOA - UNESP. Teeth with primary endodontic infection, symptomatic (n=9) or asymptomatic (n=9) and presence of visible periapical lesion on the radiograph will be included. The identification of the peptides will be done in a nanoACQUITY UPLC-Xevo QTof MS system (Waters), protein identification will be obtained using ProteinLynx Global Server software (PLGS) version 3.0 using the National Center's bacterial protein databases of Biotechnology Information. The data will be analyzed statistically with SPSS for Windows (SPSS Inc., Chicago, IL) (p <0.05). (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
YAMAMOTO, LARISSA YUMI; LOUREIRO, CAROLINE; ANGELO CINTRA, LUCIANO TAVARES; LEONARDO, RENATO DE TOLEDO; BANCI, HENRIQUE AUGUSTO; FERNANDES RIBEIRO, ANA PAULA; SIVIERI-ARAUJO, GUSTAVO; JACINTO, ROGERIO DE CASTILHO. Antibiofilm activity of laser ablation with indocyanine green activated by different power laser parameters compared with photodynamic therapy on root canals infected with Enterococcus faecalis. Photodiagnosis and Photodynamic Therapy, v. 35, . (19/17916-4, 18/18741-0)
LOUREIRO, C.; BUZALAF, M. A. R.; MORAES, F. R. N.; VENTURA, T. M. O.; PELA, V. T.; PESSAN, J. P.; JACINTO, R. C.. Quantitative proteomic analysis in symptomatic and asymptomatic apical periodontitis. International Endodontic Journal, . (19/14995-0, 18/08282-9, 18/18741-0)
CAROLINE LOUREIRO; MARÍLIA AFONSO RABELO BUZALAF; JULIANO PELIM PESSAN; FELIPE RICARDO NUNES DE MORAES; VINÍCIUS TAIOQUI PELÁ; TALITA MENDES OLIVEIRA VENTURA; ROGÉRIO DE CASTILHO JACINTO. Comparative Analysis of the Proteomic Profile of the Dental Pulp in Different Conditions. A Pilot Study. Brazilian Dental Journal, v. 31, n. 3, p. 319-336, . (18/08282-9, 19/14995-0, 18/18741-0)

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