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Inflammatory Profile and Intestinal Permeability Analysis Associated with Intestinal Microbiota after Prebiotic, Probiotic and Synbiotic Supplementation in Undernourished Rats

Grant number: 18/08873-7
Support Opportunities:Regular Research Grants
Duration: February 01, 2019 - July 31, 2022
Field of knowledge:Health Sciences - Nutrition
Principal Investigator:Claudia Cristina Alves Pereira
Grantee:Claudia Cristina Alves Pereira
Host Institution: Instituto de Saúde e Sociedade (ISS). Universidade Federal de São Paulo (UNIFESP). Campus Baixada Santista. Santos , SP, Brazil
Associated researchers:Dan Linetzky Waitzberg


The aim of the present study is to evaluate the effects of prebiotic, probiotic and synbiotic supplementation on the inflammatory profile and intestinal permeability analysis in rats submitted to undernutrition.Lewis male rats (N = 80) will be divided into 2 experimental subgroups, Nourished (n = 40) and Undernourished (n = 40). On the 10th day of the experiment, the animals will be subdivided into 8 groups, maintaining the nutrition or undernutrition condition: Negative Control (NC) (n = 10): Standard oral diet + gavage (Gv) with water; Positive Control [PC] (n = 10): Oral diet ad libitum + Gv with water; Prebiotic group [PRE] (n = 10): Oral diet ad libitum + Gv with prebiotic; Probiotic group [PRO] (n = 10): Oral diet ad libitum + Gv with probiotic. Synbiotic group [SYN] (n = 10): Oral diet ad libitum + Gv with synbiotic. At 25th day after suplementation treatment the rats will be sacrificed and blood samples will be collected for the cytokine profile (IL-1², IL-4, IL-6, IL-10, TNF-± and INF- c) and plasma levels of C-reactive protein, total proteins, albumin and pre-albumin analysis. Samples of the intestinal tissue (colon) will be designed to identify histopathological changes and gene expression. Gene expression analysis will be performed using real-time PCR by means of the genes relative quantification involved in intestinal permeability such as occlusive and adherent protein junctions (occludin, claudins 1 and 2 , E-cadherin, zonula occludente 1 and JAM-A). In order to analyze the intestinal gene expression of inflammatory markers, we will identify the intestinal gene expression profile of TLR-4, TLR-9, GPR43, MyD88, NFkB and IL-1B, IL-4, IL-6, IL-10, TNF-alpha and INF-³. From the collection of feces we will analyze the gene expression of bacteria present in the fecal microbiota and determine the concentration of short chain fatty acids (SCFA). For all the data obtained will be performed statistical tests with analysis of variance, considering p <0.05. (AU)

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