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INVESTIGATION OF ZIKA VIRUS ACTION IN CENTRAL NERVOUS SYSTEM CELLS: MODELING INFECTION IN HUMAN ASTROCYTES DERIVED FROM INDUCED PLURIPOTENT CELLS

Abstract

The Zika virus (ZIKV) is a flavivirus capable of causing infection in humans after the bite by mosquitoes of the genus Aedes contaminated with the virus. In 2015, the Brazilian strain of this virus - ZIKVBR caused an epidemic in Brazil, causing a congenital infection with the generation of several cases of cerebral malformation in infants of mothers infected by ZIKVBR. Through the study of neural progenitor cells, neurons and organoids derived from induced pluripotent stem cells (iPSC), our group and other researchers revealed the neurotrophism of ZIKVBR and helped to clarify their relationship with the Central Nervous System (CNS), showing that these cells are significantly affected by the infection. In this project, we propose to analyze biologically the effects of ZIKVBR infection in astrocyte cell culture, which are glial cells extremely important for homeostatic maintenance of neurons, also acting in inflammation of CNS. To do so, we will use astrocytes produced from human iPSCs and verify aspects of cell morphology and physiology, as well as gene and protein expression after infection with the virus. We thus hope to aid in elucidating the mechanisms used by the virus in pathogenicity caused in the CNS. (AU)

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BENAZZATO, CECILIA; RUSSO, FABIELE BALDINO; BELTRAO-BRAGA, PATRICIA CRISTINA BALEEIRO. An update on preclinical pregnancy models of Zika virus infection for drug and vaccine discovery. EXPERT OPINION ON DRUG DISCOVERY, v. 17, n. 1, . (18/16748-8, 16/02978-6)
DIAS, THOMAZ LUSCHER; SCHUCH, VIVIANE; BALEEIRO BELTRAO-BRAGA, PATRICIA CRISTINA; MARTINS-DE-SOUZA, DANIEL; BRENTANI, HELENA PAULA; FRANCO, GLORIA REGINA; NAKAYA, HELDER IMOTO. Drug repositioning for psychiatric and neurological disorders through a network medicine approach. TRANSLATIONAL PSYCHIATRY, v. 10, n. 1, . (17/25588-1, 18/03673-0, 14/10068-4, 19/00098-7, 18/16748-8)
ROSA-FERNANDES, LIVIA; CUGOLA, FERNANDA RODRIGUES; RUSSO, FABIELE BALDINO; KAWAHARA, REBECA; DE MELO FREIRE, CAIO CESAR; CORREA LEITE, PAULO EMLIO; BASSI STERN, ANA CAROLINA; ANGELI, CLAUDIA BLANES; LEAL DE OLIVEIRA, DANIELLE BRUNA; MELO, STELLA REZENDE; et al. Zika Virus Impairs Neurogenesis and Synaptogenesis Pathways in Human Neural Stem Cells and Neurons. FRONTIERS IN CELLULAR NEUROSCIENCE, v. 13, . (16/02978-6, 14/06863-3, 13/08844-3, 18/16748-8, 11/20683-0, 15/02866-0)
YDE OHKI, CRISTINE MARIE; BENAZZATO, CECILIA; RUSSO, FABIELE BALDINO; BALEEIRO BELTRAO-BRAGA, PATRICIA CRISTINA. Developing animal models of Zika virus infection for novel drug discovery. EXPERT OPINION ON DRUG DISCOVERY, v. 14, n. 6, . (16/02978-6, 18/16748-8)

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