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Bioprospecting, characterization and optimization of Brazilian microalgal strains for CO2 biofixation and bioproducts of commercial importance

Grant number: 18/07988-5
Support type:Research Projects - Thematic Grants
Duration: November 01, 2018 - October 31, 2023
Field of knowledge:Biological Sciences - Botany - Applied Botany
Principal researcher:Ana Teresa Lombardi
Grantee:Ana Teresa Lombardi
Home Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
Pesquisadores principais:
Armando Augusto Henriques Vieira ; Maria da Graça Gama Melão
Assoc. researchers:Adriano Polpo de Campos ; Antonio Carlos Luperni Horta ; Artur Placeres Neto ; Camila Manoel Crnkovic ; Christopher Parrish ; Clovis Wesley Oliveira de Souza ; Denise Bevilaqua ; Inessa Lacativa Bagatini ; José Bonomi Barufi ; Juergen Polle ; Leonardo Rubi Rorig ; Mathias Ahii Chia ; Melina Guimaraes ; Ondrej Prasil ; Paula Cristina Garcia Manoel Crnkovic
Associated scholarship(s):20/15953-7 - The role of NaCL on the physiology and biomolecules production in the microalga Curvastrum pantanale, BP.IC
20/12575-1 - Characterization of the cyanobacteria Rhabdoderma lineare: a physiological, biochemical and metabolic approach, BP.IC
20/15688-1 - Defining the best cultivation systems for scaling up different microalgae species (species selected in the previous stages of the thematic project), BP.PD
+ associated scholarships 20/12508-2 - Prospection and physiology of Brazilian microalgae: biochemical potential, biological activity and metabolomic analysis, BP.DD
20/04619-9 - Biotechnological strategy for inducing the synthesis of biomolecules in Ankistrodesmus fusiformis: a chemical stress study, BP.IC
19/07579-0 - Characterization of microalgae for CO2 biofixation and manipulation of its biochemical composition for industrial applications, BP.DR
19/16273-2 - The production of biomolecules in microlagae: process improvement in laboratory and large scales, BP.DD
19/03177-5 - Applications of the biodiversity of native microalgae in Brazil: importance of taxonomic, systematic and molecular phylogeny., BP.PD
19/14152-3 - The effects of manipulation of environmental conditions in photosynthesis and value-added biomolecules yield in Kirchneriella contorta, BP.IC
19/01930-8 - Influence of the physiological state on the freezing of freshwater microalgae: obtaining the best viability rates and measurement of functional stability of the samples after freezing, BP.PD
19/02503-6 - Copper as biomolecule manipulating agent in microalgae cultures (scales 100 to 400 L), BP.TT - associated scholarships


This proposal focuses on microalgae physiology and biotechnology, starting from test tubes towards scaling up, in search for robust microalgae and their products that may be of commercial interest. Our goal is to identify strains that present high biomass yields and produce intracellular biomolecules with antioxidant, antibacterial, lipid, carbohydrate, amino acid, fatty acid, phytosterols and/or terpenoid properties, and/or extracellular polysaccharides with tensoactive properties. Our project has two main objectives, the prospecting of biomolecules with CO2 biofixation, and the production of biochemically characterized biomass. We include here the identification of strains from the Freshwater Microalgae Culture Collection (UFSCar), and the study of cryopreservation in light of physiological characterization as a guarantee for further germination. We will prospect about 300 strains, establish a library of extracts, and select strains for scaling up (100 - 1000 L). Due to the interdisciplinary nature of our proposed project, we integrate a multidisciplinary and interinstitutional team into cohesive working groups. In the laboratory, strains that produce exopolysaccharides/mucilage capsules will be tested for tensing properties. The biofixation of CO2 permeates all stages of this proposal as it is the basis for photosynthetic metabolism. In this regard, we intend to conclude with a model that facilitates the quantitation of the fixed gas, currently an unresolved issue. In relation to biomolecules, we will prospect for antioxidant, antibacterial, total lipids, proteins and carbohydrates, amino acids and fatty acids profiles, phytosterols, terpenes and energy potential. We will start the project with the goal of screening 60 strains/year. From the total of 300 strains, we will down-select to the 10 most promising strains based on CO2 biofixation, robustness in culture and biomolecules of interest. For these down-selected strains, we will apply biochemical manipulation through abiotic stress to increase the synthesis and accumulation of intracellular biomolecules. With the triad biofixation of CO2, robustness in cultivation, and biomolecules we will scale up to 100 - 500 L (greenhouse), and from these we will go to 1000 L cultures (planar and thin layer photobioreactors) with strains of best performance, which will be investigated through transcriptomics to learn the about metabolic routes of biomolecule and biomass production. The results obtained will be an important contribution to sectors of the society interested in the production of microalgae and/or biofixation of CO2, and to those that need to make such interest feasible, adding value to the biomass. In addition, we will contribute to basic information to phycology and keep a library of extracts with biologically and chemically characterized strains of microalgae available to the community. (AU)

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Scientific publications (11)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE ABREU, CINTHIA BRUNO; GEBARA, RENAN CASTELHANO; DOS REIS, LARISSA LUIZA; ROCHA, GISELI SWERTS; GONCALVES ALHO, LAYS DE OLIVEIRA; ALVARENGA, LAIS MENDES; VIRTUOSO, LUCIANO SINDRA; ASSIS, MARCELO; MANSANO, ADRISLAINE DA SILVA; LONGO, ELSON; GAMA MELAO, MARIA DA GRACA. Toxicity of alpha-Ag2WO4 microcrystals to freshwater microalga Raphidocelis subcapitata at cellular and population levels. Chemosphere, v. 288, n. 2 FEB 2022. Web of Science Citations: 0.
STABLEIN, MICHAEL J.; BARACHO, DOUGLAS H.; WATSON, JAMISON T.; SILVA, JAQUELINE C.; ZHANG, YUANHUI; LOMBARDI, ANA T. Microalgal photosynthetic inhibition and mixotrophic growth in Post Hydrothermal Liquefaction Wastewater (PHW). ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, v. 60, DEC 2021. Web of Science Citations: 0.
GEBARA, RENAN CASTELHANO; GONCALVES ALHO, LAYS DE OLIVEIRA; DE ABREU, CINTHIA BRUNO; MANSANO, ADRISLAINE DA SILVA; MOREIRA, RAQUEL APARECIDA; ROCHA, GISELI SWERTS; GAMA MELAO, MARIA DA GRACA. Toxicity and Risk Assessment of Zinc and Aluminum Mixtures to Ceriodaphnia silvestrii (Crustacea: Cladocera). Environmental Toxicology and Chemistry, v. 40, n. 10 SEP 2021. Web of Science Citations: 0.
ROCHA, GISELI SWERTS; LOMBARDI, ANA TERESA; ESPINDOLA, EVALDO L. G. Combination of P-limitation and cadmium in photosynthetic responses of the freshwater microalga Ankistrodesmus densus (Chlorophyceae). Environmental Pollution, v. 275, APR 15 2021. Web of Science Citations: 0.
CAMARGO, EDUARDO CAFFAGNI; ROSSI, RAQUEL ANDRADE; SILVA, JAQUELINE CARMO; MIWA, ADRIANA CRISTINA POLI; PRASIL, ONDREJ; CALIJURI, MARIA DO CARMO; LOMBARDI, ANA TERESA. Comparing pulse amplitude modulated (PAM) fluorometry with radiocarbon technique for determination of inorganic carbon fixation in Chlorella vulgaris (Trebouxiophyceae, Chlorophyta). European Journal of Phycology, APR 2021. Web of Science Citations: 0.
BARRETO, DANIELA MARIANO; TONIETTO, ALESSANDRA EMANUELE; LOMBARDI, ANA TERESA. Environmental concentrations of copper nanoparticles affect vital functions in Ankistrodesmus densus. AQUATIC TOXICOLOGY, v. 231, FEB 2021. Web of Science Citations: 0.
DOS REIS, LARISSA LUIZA; GONCALVES ALHO, LAYS DE OLIVEIRA; DE ABREU, CINTHIA BRUNO; GAMA MELA, MARIA DA GRACA. Using multiple endpoints to assess the toxicity of cadmium and cobalt for chlorophycean Raphidocelis subcapitata. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v. 208, JAN 15 2021. Web of Science Citations: 3.
RAFAEL BARTY DEXTRO. Growth dynamic on a co-cultivation of two Chlorophyta microalgae exposed to copper. Acta Limnol. Bras., v. 33, p. -, 2021.
GONCALVES ALHO, LAYS DE OLIVEIRA; SOUZA, JAQUELINE PEROLA; ROCHA, GISELI SWERTS; MANSANO, ADRISLAINE DA SILVA; LOMBARDI, ANA TERESA; SARMENTO, HUGO; GAMA MELAO, MARIADA GRACA. Photosynthetic, morphological and biochemical biomarkers as tools to investigate copper oxide nanoparticle toxicity to a freshwater chlorophyceae. Environmental Pollution, v. 265, n. A OCT 2020. Web of Science Citations: 9.
GEBARA, RENAN CASTELHANO; GONCALVES ALHO, LAYS DE OLIVEIRA; ROCHA, GISELI SWERTS; MANSANO, ADRISLAINE SILVA; GAMA MELAO, MARIA GRACA. Zinc and aluminum mixtures have synergic effects to the algae Raphidocelis subcapitata at environmental concentrations. Chemosphere, v. 242, MAR 2020. Web of Science Citations: 0.
CANDIDO, CAMILA; LOMBARDI, ANA TERESA. Mixotrophy in green microalgae grown on an organic and nutrient rich waste. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, v. 36, n. 2 JAN 18 2020. Web of Science Citations: 0.

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