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Use of fluorescence spectroscopy on detection and control of contamination in food


Cardboard, spoiled meat, chemical additives in concentrations above of permitted, microorganisms, pesticides, antibiotics, zoonoses, etc. What is the quality of the foods we consume? Food poisoning is a much bigger problem for governments and for the food industry today, compared to the last decade. The World Health Organization (WHO) reports indicate that all over the world, millions of people suffer from diseases caused by contaminated food. Food contamination is generally defined as foods that are spoiled or tainted because they either contain microorganisms, such as bacteria or parasites, or toxic substances that make them unfit for consumption. In addition, different chemical mechanisms are responsible for the oxidation of fats and oils during processing, storage, and cooking generating high levels of reactive oxygen species (ROS) and free radicals. The goal of this project is the utilization of fluorescence spectroscopic in detection of contaminants (microrganisms, pesticides and antibiotics) and oxidative processes that deteriorate the quality of food. Fluorescence spectroscopy is a technique in which the excitation of a molecule is obtained through the absorption of photons. With this technique it is possible to quickly determine the biochemical and biophysical properties of foods. We can cite some important properties of fluorescence when compared with other analytical methods as: fast response, high sensitivity, low detection limits, selectivity, instrumental simplicity and low cost of maintenance and analysis. Through fluorescence measurements it is also possible to determine the importance and specific contribution of the singlet oxygen in the oxidation of foods. The deep scientific knowledge of the formation, reactions, mechanisms and kinetics of generation/extinction of singlet oxygen, can significantly improve the quality of foods, reducing oxidation during processing and storage. With the approval of this project, it will be possible to make improvements in the fluorimeter Jobin Yvon Fluorolog 3, acquired with Fapesp 06/06591-7 process by adding a module of acquisition in the infrared region and software update. It will also be possible to purchase a new pulsed excitation source for the PicoQuant's lifetime system acquired in the FAPESP 10/16544-1 process. These actions will allow the expansion of the possibility of measurement and new collaborations. (AU)

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Scientific publications (7)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
COURROL, LILIA CORONATO; DE OLIVEIRA SILVA, FLAVIA RODRIGUES; MASILAMANI, VALDIVEL. SARS-CoV-2, hemoglobin and protoporphyrin IX: Interactions and perspectives. Photodiagnosis and Photodynamic Therapy, v. 34, . (17/23686-6)
GONCALVES, KARINA DE OLIVEIRA; VIEIRA, DANIEL PEREZ; LEVY, DEBORA; BYDLOWSKI, SERGIO PAULO; COURROL, LILIA CORONATO. Uptake of silver, gold, and hybrids silver-iron, gold-iron and silver-gold aminolevulinic acid nanoparticles by MCF-7 breast cancer cells. Photodiagnosis and Photodynamic Therapy, v. 32, . (14/50983-3, 17/23686-6, 14/06960-9)
BORGES LOPES, CARLA REGINA; SANTOS JUNIOR, DARIO; DE OLIVEIRA SILVA, FLAVIA RODRIGUES; COURROL, LILIA CORONATO. High-sensitivity Hg2+ sensor based on the optical properties of silver nanoparticles synthesized with aqueous leaf extract of Mimusops coriacea. APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING, v. 127, n. 4, . (17/23686-6)
COURROL, LILIA CORONATO; VALLIM, MARCELO AFONSO. Characterization of chicken meat contaminated with Salmonella by fluorescence spectroscopy. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, v. 261, . (15/04400-9, 17/23686-6)
DA SILVA, RAFAEL LEONARDO C. G.; GONCALVES, KARINA DE OLIVEIRA; COURROL, LILIA CORONATO; CASELI, LUCIANO. Study of the interactions of gold nanoparticles functionalized with aminolevulinic acid in membrane models. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 205, . (19/03239-0, 18/00291-9, 17/23686-6, 18/22214-6)
SANTOS, NOEMY R.; KUNZEL, ROSELI; FREITAS, MARCELO B.; LEVENHAGEN, RONALDO S.; MARQUES, ANA PAULA DE A.; COURROL, LILIA C.. Raman and Fluorescence Profiles Modifications of Muscular and Adipose Tissues Exposed to Low Energy X-ray Beams. Applied Spectroscopy, v. 75, n. 9, . (17/23686-6)
COURROL, LILIA CORONATO; VALLIM, MARCELO AFONSO. Spectroscopic Analysis of Chicken Meat Contaminated with E. coli, Salmonella, and Campylobacter. FOOD ANALYTICAL METHODS, v. 14, n. 3, p. 512-524, . (17/23686-6, 15/04400-9)

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