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Proteomic and peptidomic analysis of snake venom effect on cancer cell lines and mouse brain tissues

Abstract

Toxic substances and secretions of animals such as snakes, spiders and scorpions have been used in traditional medicine for the treatment of several diseases. Despite the inherent difficulty and high economic risk in the discovery and development of new drugs from natural products, the development and rapid advances of technologies such as mass spectrometry-based proteome analysis has allowed researchers to advance in a more focused characterization for the determination of key molecular targets in several diseases.There are several works in the literature describing the biochemical and physiological analyzes characterizing the snake venom effect on organs, tissues and cells, showing their toxic and cytotoxic effects as well as the induction of biochemical changes and imbalances. However, there are few or almost no studies characterizing the proteomic and peptidomic profiles of the effect of venom on organs, tissues, and cells aiming at the evaluating the affected molecular targets.Based on the hypothesis that low or sub-lethal doses of snake venom are capable to modulate protein expression in cells and cerebral tissues, this project aims to analyze the comparative proteomic and peptidomic profiles of different cancer cell lines (MCF7, MDA-MB231, A204, SK-MEL-28, HeLa, PANC-1, and Y1) and normal cells (HEK293, AsPC-1, HACAT, and Y1-RasKO) treated or not with Bothrops jararaca snake venom and cerebral tissues (cortex, cerebellum, hippocampus, hypothalamus and striatum) of mice treated or not with Crotalus durissus terrificus snake venom in order to correlate them with cellular morphological and histological data of the damage caused by the venom. These data may suggest new pharmacological targets and novel therapeutic approaches (i.e. targeting proteins initially affected by the venom) for the envenoming treatment by the studied venoms. (AU)

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Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
NOVAES, TATIANE FERNANDES; JORDAO, MAISA CAMILLO; BONACINA, CARLOS FELIPE; VERONEZI, ANDRE OSWALDO; RODRIGUES DE ARAUJO, CARLOS ARIEL; OLEGARIO, ISABEL CRISTINA; DE OLIVEIRA, DANIELE BOINA; USHAKOVA, VERANIKA; BIRBRAIR, ALEXANDER; PALACIO, DANIELLE DA COSTA; et al. COVID-19 pandemic impact on dentists in Latin America's epicenter: SAo-Paulo, Brazil. PLoS One, v. 16, n. 8, . (13/07467-1, 17/17943-6)
NAVARRO, MARINA V.; CHAVES, ALISON F. A.; CASTILHO, DANIELE G.; CASULA, ISIS; CALADO, JULIANA C. P.; CONCEICAO, PALLOMA M.; IWAI, LEO K.; DE CASTRO, BEATRIZ F.; BATISTA, WAGNER L.. Effect of Nitrosative Stress on theS-Nitroso-Proteome ofParacoccidioides brasiliensis. FRONTIERS IN MICROBIOLOGY, v. 11, . (17/04592-0, 13/07467-1, 16/04000-3, 15/09654-9, 17/17943-6)
MONTONI, FABIO; ANDREOTTI, DIANA ZUKAS; DOS SANTOS EICHLER, ROSANGELA APARECIDA; SANTOS, WELLINGTON DA SILVA; KISAKI, CAROLINA YUKIKO; SUEHIRO ARCOS, STEPHANIE SANTOS; LIMA, ISMAEL FEITOSA; MALTAURO SOARES, MAGNA APARECIDA; NISHIYAMA-JR, MILTON YUTAKA; NAVA-RODRIGUES, DANIEL; et al. The impact of rattlesnake venom on mice cerebellum proteomics points to synaptic inhibition and tissue damage. JOURNAL OF PROTEOMICS, v. 221, . (17/17943-6, 13/07467-1, 16/04000-3, 15/50040-4, 17/06496-9)
FIGUEIREDO, D. A. L.; SANTOS, W. S.; MONTONI, FABIO; IWAI, LEO KEI; SILVA JUNIOR, I, P.. Toposome: Source of antimicrobial molecules in the gonads of the sea urchin Lytechinus variegatus (Lamarck, 1816). FISH & SHELLFISH IMMUNOLOGY, v. 109, p. 51-61, . (17/17943-6, 16/04000-3)
KISAKI, CAROLINA YUKIKO; ARCOS, STEPHANIE SANTOS SUEHIRO; MONTONI, FABIO; DA SILVA SANTOS, WELLINGTON; CALACINA, HAMIDA MACEDO; LIMA, ISMAEL FEITOSA; CAJADO-CARVALHO, DANIELA; FERRO, EMER SUAVINHO; NISHIYAMA-JR, MILTON YUTAKA; IWAI, LEO KEI. Bothrops Jararaca Snake Venom Modulates Key Cancer-Related Proteins in Breast Tumor Cell Lines. TOXINS, v. 13, n. 8, . (17/06496-9, 13/07467-1, 16/04000-3, 17/17943-6)
MOREIRA, RAFAEL J.; CASTRO, ERIQUE; OLIVEIRA, TIAGO E.; BELCHIOR, THIAGO; PEIXOTO, ALBERT S.; CHAVES-FILHO, ADRIANO B.; MORENO, MAYARA F.; LIMA, JANAYNA D.; YOSHINAGA, MARCOS; MIYAMOTO, SAYURI; et al. Lipoatrophy-Associated Insulin Resistance and Hepatic Steatosis are Attenuated by Intake of Diet Rich in Omega 3 Fatty Acids. MOLECULAR NUTRITION & FOOD RESEARCH, v. 64, n. 7, . (15/19530-5, 17/17943-6, 14/06863-3, 16/04000-3, 13/07937-8, 13/07467-1, 18/15549-1)

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