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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Optical Biomarkers of Serous and Mucinous Human Ovarian Tumor Assessed with Nonlinear Optics Microscopies

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Autor(es):
Adur, Javier [1, 2] ; Pelegati, Vitor B. [1] ; de Thomaz, Andre A. [1] ; Baratti, Mariana O. [3] ; Almeida, Diogo B. [1] ; Andrade, L. A. L. A. [4] ; Bottcher-Luiz, Fatima [5] ; Carvalho, Hernandes F. [3, 6] ; Cesar, Carlos L. [1, 3]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] State Univ Campinas UNICAMP, Inst Phys Gleb Wataghin, Opt & Photon Res Ctr CEPOF, Biophoton Grp, Sao Paulo - Brazil
[2] Natl Univ Entre Rios, Sch Bioengn, Microscopy Lab Appl Mol & Cellular Studies, Oro Verde, Entre Rios - Argentina
[3] INFABiC Natl Inst Sci & Technol Photon Appl Cell, Sao Paulo - Brazil
[4] State Univ Campinas UNICAMP, Fac Med Sci, Dept Pathol Anat, Sao Paulo - Brazil
[5] State Univ Campinas UNICAMP, Fac Med Sci, Dept Obstet & Gynecol, Sao Paulo - Brazil
[6] State Univ Campinas UNICAMP, Dept Anat Cell Biol Physiol & Biophys, Inst Biol, Sao Paulo - Brazil
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 7, n. 10 OCT 8 2012.
Citações Web of Science: 26
Resumo

Background: Nonlinear optical (NLO) microscopy techniques have potential to improve the early detection of epithelial ovarian cancer. In this study we showed that multimodal NLO microscopies, including two-photon excitation fluorescence (TPEF), second-harmonic generation (SHG), third-harmonic generation (THG) and fluorescence lifetime imaging microscopy (FLIM) can detect morphological and metabolic changes associated with ovarian cancer progression. Methodology/Principal Findings: We obtained strong TPEF + SHG + THG signals from fixed samples stained with Hematoxylin \& Eosin (H\&E) and robust FLIM signal from fixed unstained samples. Particularly, we imaged 34 ovarian biopsies from different patients (median age, 49 years) including 5 normal ovarian tissue, 18 serous tumors and 11 mucinous tumors with the multimodal NLO platform developed in our laboratory. We have been able to distinguish adenomas, borderline, and adenocarcinomas specimens. Using a complete set of scoring methods we found significant differences in the content, distribution and organization of collagen fibrils in the stroma as well as in the morphology and fluorescence lifetime from epithelial ovarian cells. Conclusions/Significance: NLO microscopes provide complementary information about tissue microstructure, showing distinctive patterns for serous and mucinous ovarian tumors. The results provide a basis to interpret future NLO images of ovarian tissue and lay the foundation for future in vivo optical evaluation of premature ovarian lesions. (AU)

Processo FAPESP: 08/57906-3 - Instituto Nacional de Fotônica Aplicada à Biologia Celular - INFABIC
Beneficiário:Hernandes Faustino de Carvalho
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 10/12565-4 - Microscopia fotônica integrada e ferramentas de manipulação de células para o estudo de processos
Beneficiário:Javier Fernando Adur
Linha de fomento: Bolsas no Brasil - Pós-Doutorado