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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Environmental influences on and antimicrobial activity of the skin microbiota of Proceratophrys boiei (Amphibia, Anura) across forest fragments

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Autor(es):
Assis, Ananda B. [1] ; Bevier, Catherine R. [2] ; Chaves Barreto, Cristine [3] ; Arturo Navas, Carlos [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Biosci, Dept Physiol, Sao Paulo - Brazil
[2] Colby Coll, Dept Biol, Waterville, ME 04901 - USA
[3] Univ Catolica Brasilia, Grad Program Genom Sci & Biotechnol, Brasilia, DF - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: ECOLOGY AND EVOLUTION; v. 10, n. 2 JAN 2020.
Citações Web of Science: 0
Resumo

The composition of the skin microbiota of amphibians is related to the biology of host species and environmental microbial communities. In this system, the environment serves as a microbial source and can modulate the hosted community. When habitats are fragmented and the environment disturbed, changes in the structure of this microbial community are expected. One important potential consequence of fragmentation is a compromised protective function of the microbiota against pathogenic microorganisms. In this study, the skin microbiota of the amphibian Proceratophrys boiei was characterized, evaluated for relationships with environmental variables and environmental sources of microbial communities, and its diversity evaluated for frog populations from fragmented and continuous forests. In addition, the antimicrobial activity of this skin community was studied in frogs from both forest types. Culture methods and 16S rRNA high-throughput gene sequencing were used to characterize the microbial community and demonstrated that the skin microbiota of P. boiei is more closely related to the soil microbial communities than those inhabiting water bodies or fragment matrix, the unforested area around the forested fragment. The microbial diversity and abundance of P. boiei skin microbiota are different between continuous forests and fragments. This community is correlated with environmental variables, especially with temperature of microhabitat and distance to human dwelling. All individuals of P. boiei harbored bacteria capable of inhibiting the growth of pathogenic bacteria and different strains of the pathogenic fungus Batrachochytrium dendrobatidis, and a total of 27 bacterial genera were detected. The results of this study indicate that the persistence of populations of this species will need balanced and sustained interactions among host, microorganisms, and environment. (AU)

Processo FAPESP: 11/50079-7 - Efeitos da fragmentação florestal sobre a microbiota cutânea e secreção dérmica de Proceratophrys boiei (Amphibia, Anura)
Beneficiário:Carlos Arturo Navas Iannini
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 10/52075-6 - Microbiotas cutâneas e secreções dérmicas de Proceratophrys boiei (amphibia,anura) em fragmentos de Floresta Atlântica
Beneficiário:Ananda Brito de Assis
Linha de fomento: Bolsas no Brasil - Doutorado