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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Production of whey protein isolate - gellan microbeads for encapsulation and release of flaxseed bioactive compounds

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Autor(es):
Kuhn, Katia Regina [1] ; Drummond e Silva, Fernanda Guimaraes [2] ; Netto, Flavia Maria [2] ; da Cunha, Rosiane Lopes [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Dept Food Engn, Fac Food Engn, UNICAMP, BR-13083862 Campinas, SP - Brazil
[2] Univ Estadual Campinas, Dept Food & Nutr, Fac Food Engn, UNICAMP, BR-13083862 Campinas, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Journal of Food Engineering; v. 247, p. 104-114, APR 2019.
Citações Web of Science: 2
Resumo

Production of 1.5% (w/v) whey protein isolate (WPI) - 0.1, 0.3 or 0.5% (w/v) gellan gum microbeads from extrusion of the oil-in-water (O/W) emulsions into a 0.56% (w/v) calcium chloride (CaCl2) solution was evaluated to encapsulate flaxseed oil (15% v/v) and protein hydrolysate (FPH) (0, 0.25 or 0.5% w/v). Microgels resistance and controlled release of oil and FPH were also investigated. Microscopic images showed few free oil droplets and a prevailing presence of gellan on the external surface of the microbeads, indicating that oil and FPH were encapsulated. Microbeads produced at higher gellan concentrations (0.3 or 0.5% w/v) showed a more regular and spherical morphology. However a significant decrease in microbeads size (from similar to 55 mu m to similar to 50 mu m) and an increase in the polydispersity were observed with the FPH addition, which can be a consequence of the formation of a more dense biopolymers network. FPH presence (0.25% w/v) decreased the viscosity and shear thinning behavior of microbeads suspensions (10-90% w/v), which could be partly attributed to the smaller size of particles. The microbeads suspensions were stable at different salt concentrations (0.56, 1.11 or 2.22% w/v) regarding their shape, not releasing the encapsulated oil. 1.5% (w/v) WPI similar to 0.3% (w/v) gellan microbeads were resistant to simulated gastric conditions, but did not resist to intestinal conditions. Our results show that these microgels are adequate to encapsulate bioactive compounds to be released in the small intestine, passing intact in the stomach, which makes the process attractive in order to maintain the bioavailability and functionality of such compounds. (AU)

Processo FAPESP: 08/57906-3 - Instituto Nacional de Fotônica Aplicada à Biologia Celular - INFABIC
Beneficiário:Hernandes Faustino de Carvalho
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 09/54137-1 - EMUS: aquisição de um analisador de distribuição de tamanho de partículas, um medidor de potencial zeta, um spray dryer e um sistema de cromatografia líquida de ultra alta pressão / espectrômetro de massa
Beneficiário:Miriam Dupas Hubinger
Linha de fomento: Auxílio à Pesquisa - Programa Equipamentos Multiusuários