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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Potential application of laccase from Pycnoporus sanguineus in methanol/O-2 biofuel cells

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Autor(es):
Aquino Neto, S. [1] ; Zimbardi, A. L. R. L. [1] ; Cardoso, F. P. [1] ; Crepaldi, L. B. [1] ; Minteer, S. D. [2, 3] ; Jorge, J. A. [4] ; Furriel, R. P. M. [1] ; De Andrade, A. R. [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, BR-14040901 Ribeirao Preto, SP - Brazil
[2] Univ Utah, Dept Chem, Salt Lake City, UT 84112 - USA
[3] Univ Utah, Dept Mat Sci & Engn, Salt Lake City, UT 84112 - USA
[4] Univ Sao Paulo, Dept Biol, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-14040901 Ribeirao Preto, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Journal of Electroanalytical Chemistry; v. 765, p. 2-7, MAR 15 2016.
Citações Web of Science: 8
Resumo

This paper describes the preparation and characterization of biocathodes for a methanol/O-2 biofuel cell using the crude extract and a purified laccase from a strain of Pycnoporus sanguineus (PyS). The biocathode assembly with mediated electron transfer was achieved by immobilizing either the enzymes in the crude extract or the purified laccase with polyamidoamine (PAMAM) dendrimers on top of a polypyrrole matrix containing entrapped 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) species as the redox mediator. Carbon nanotubes modified with anthracene moieties were used to orientate the purified laccase and allow for direct bioelectrocatalytic 02 reduction. The kinetic characterization of the biocathodes showed that samples prepared with either the crude extract or the purified laccase retained ABTS oxidation activity after the immobilization procedure. The biofuel cell tests showed that the biocathodes were able to furnish maximum power densities of 51.2 and 99.8 mu W cm(-2) for the samples prepared with the crude extract and the purified enzyme, respectively. Moreover, it was observed that both biocathodes tested displayed a very similar loss in power performance as a function of storage time, reaching 60% of the initial activity after 60 days. Overall, the laccase-based biocathodes prepared either with the crude extract or with the purified enzyme are able to provide electrochemically active and stable biomaterials. (C) 2015 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 12/10667-0 - Preparação de nanocatalisadores híbridos contendo enzimas, nanotubos de carbono e nanopartículas metálicas para biocélula a combustível EtOH/O2
Beneficiário:Sidney de Aquino Neto
Linha de fomento: Bolsas no Brasil - Pós-Doutorado