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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Trypanosoma cruzi mitochondrial tryparedoxin peroxidase is located throughout the cell and its pull down provides one step towards the understanding of its mechanism of action

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Autor(es):
Peloso, E. F. [1, 2] ; Dias, L. [2] ; Queiroz, R. M. L. [3] ; Leme, A. F. P. Paes [4] ; Pereira, C. N. [2] ; Carnielli, C. M. [4] ; Werneck, C. C. [2] ; Sousa, M. V. [3] ; Ricart, C. A. O. [3] ; Gadelha, F. R. [2]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Fac Sao Lucas, Porto Velho, Rondonia - Brazil
[2] Univ Estadual Campinas, Dept Bioquim & Biol Tecidual, Campinas, SP - Brazil
[3] Univ Brasilia, Inst Ciencias Biol, Lab Bioquim & Quim Prot, Brasilia, DF - Brazil
[4] Lab Nacl Biociencias, Ctr Nacl Pesquisa Energ & Mat, Campinas, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS; v. 1864, n. 1, p. 1-10, JAN 2016.
Citações Web of Science: 4
Resumo

Trypanosoma cruzi depends on the effectiveness of redox metabolism to survive and ensure infection in the host. Homeostasis of redox metabolism in T. cruzi is achieved by the actions of several proteins that differ in many aspects from host proteins. Although extensive research has been performed examining hydroperoxide cytosolic antioxidant defense centered on trypanothione, the mechanisms of mitochondrial antioxidant defense are not yet known. The aim of this study was to elucidate the partners of TcMPx antioxidant pathway and to determine the influence of the cellular context (physiological versus oxidative stress). Through co-precipitation coupled with a mass spectrometry approach, a variety of proteins were detected under physiological and oxidative stress conditions. Interestingly, functional category analysis of the proteins identified under physiological conditions showed that they were involved in the stress response, oxidoreduction, thiol transfer, and metabolic processes; this profile is distinct under oxidative stress conditions likely due to structural alterations. Our findings help to elucidate the reactions involving TcMPx and most importantly also reveal that this protein is present throughout the cell and that its interaction partners change following oxidative stress exposure. The involvement and significance of the proteins found to interact with TcMPx and other possible functions for this protein are discussed widening our knowledge regarding T. cruzi mitochondrial antioxidant defenses. (C) 2015 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 08/57906-3 - Instituto Nacional de Fotônica Aplicada à Biologia Celular - INFABIC
Beneficiário:Hernandes Faustino de Carvalho
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 11/19872-2 - Determinação do interatoma, caracterização funcional e outras localizações intracelulares da triparedoxina peroxidase mitocondrial do Trypanosoma Cruzi
Beneficiário:Eduardo de Figueiredo Peloso
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 11/20084-9 - Caracterização funcional e localização intracelular de componentes do sistema antioxidante mitocondrial e nuclear do Trypanosoma cruzi dependentes ou não da tripanotiona
Beneficiário:Fernanda Ramos Gadelha
Linha de fomento: Auxílio à Pesquisa - Regular