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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Reduced graphene oxide induces transient blood-brain barrier opening: an in vivo study

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Autor(es):
Padilha Mendonca, Monique Culturato [1, 2] ; Soares, Edilene Siqueira [2] ; de Jesus, Marcelo Bispo [2] ; Ceragioli, Helder Jose [3] ; Ferreira, Monica Siqueira [4] ; Catharino, Rodrigo Ramos [4] ; da Cruz-Hoefling, Maria Alice [1, 2]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Dept Pharmacol, Fac Med Sci, Campinas, SP - Brazil
[2] Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, Campinas, SP - Brazil
[3] Univ Estadual Campinas, Fac Elect & Comp Engn, Dept Semicond Instruments & Photon, Campinas, SP - Brazil
[4] Univ Estadual Campinas, Dept Med & Expt Surg, Fac Med Sci, Campinas, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF NANOBIOTECHNOLOGY; v. 13, OCT 30 2015.
Citações Web of Science: 31
Resumo

Background: The blood-brain barrier (BBB) is a complex physical and functional barrier protecting the central nervous system from physical and chemical insults. Nevertheless, it also constitutes a barrier against therapeutics for treating neurological disorders. In this context, nanomaterial-based therapy provides a potential alternative for overcoming this problem. Graphene family has attracted significant interest in nanomedicine because their unique physicochemical properties make them amenable to applications in drug/gene delivery and neural interface. Results: In this study, reduced graphene oxide (rGO) systemically-injected was found mainly located in the thalamus and hippocampus of rats. The entry of rGO involved a transitory decrease in the BBB paracellular tightness, as demonstrated at anatomical (Evans blue dye infusion), subcellular (transmission electron microscopy) and molecular (junctional protein expression) levels. Additionally, we examined the usefulness of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) as a new imaging method for detecting the temporal distribution of nanomaterials throughout the brain. Conclusions: rGO was able to be detected and monitored in the brain over time provided by a novel application for MALDI-MSI and could be a useful tool for treating a variety of brain disorders that are normally unresponsive to conventional treatment because of BBB impermeability. (AU)

Processo FAPESP: 08/57906-3 - Instituto Nacional de Fotônica Aplicada à Biologia Celular - INFABIC
Beneficiário:Hernandes Faustino de Carvalho
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 11/50400-0 - Metabolismo energético, estado redox e funcionalidade mitocondrial na morte celular e em desordens cardiometabólicas e neurodegenerativas
Beneficiário:Aníbal Eugênio Vercesi
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 12/24782-5 - Óxido de grafeno e sistema nervoso central: avaliação dos efeitos na barreira hematoencefálica e perfil nanotoxicológico
Beneficiário:Monique Culturato Padilha Mendonça
Linha de fomento: Bolsas no Brasil - Doutorado