Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Endophytic fungi from the Amazonian plant Paullinia cupana and from Olea europaea isolated using cassava as an alternative starch media source

Texto completo
Autor(es):
Sia, Eliandra de Freitas [1] ; Marcon, Joelma [2] ; Luvizotto, Danice Mazzer [2] ; Quecine, Maria Carolina [2] ; Tsui, Sarina [2] ; Pereira, Jose Odair [1] ; Pizzirani-Kleiner, Aline Aparecida [2] ; Azevedo, Joao Lucio [1, 2]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Fed Amazonas UFAM, BR-69077000 Manaus, Amazonas - Brazil
[2] Univ Sao Paulo, Dept Genet, Escola Super Agr Luiz de Queiroz, BR-13400970 Piracicaba, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: SPRINGERPLUS; v. 2, 2013.
Citações Web of Science: 5
Resumo

Endophytic fungi live inside plants, apparently do not cause any harm to their hosts and may play important roles in defense and growth promotion. Fungal growth is a routine practice at microbiological laboratories, and the Potato Dextrose Agar (PDA) is the most frequently used medium because it is a rich source of starch. However, the production of potatoes in some regions of the world can be costly. Aiming the development of a new medium source to tropical countries, in the present study, we used leaves from the guarana (a tropical plant from the Amazon region) and the olive (which grows in subtropical and temperate regions) to isolate endophytic fungi using PDA and Manihot Dextrose Agar (MDA). Cassava (Manihot esculenta) was evaluated as a substitute starch source. For guarana, the endophytic incidence (EI) was 90% and 98% on PDA and MDA media, respectively, and 65% and 70% for olive, respectively. The fungal isolates were sequenced using the ITS-rDNA region. The fungal identification demonstrated that the isolates varied according to the host plant and media source. In the guarana plant, 13 fungal genera were found using MDA and six were found using PDA. In the olive plant, six genera were obtained using PDA and 4 were obtained using MDA. The multivariate analysis results demonstrated the highest fungal diversity from guarana when using MDA medium. Interestingly, some genera were isolated from one specific host or in one specific media, suggesting the importance of these two factors in fungal isolation specificity. Thus, this study indicated that cassava is a feasible starch source that could serve as a potential alternative medium to potato medium. (AU)

Processo FAPESP: 10/15192-4 - Estudo da diversidade de bactérias endofíticas cultiváveis associadas ao guaranazeiro [Paullinia cupana (Mart.) ducke.] visando o controle de antracnose
Beneficiário:Sarina Tsui
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 09/53376-2 - Microrganismos associados ao guaranazeiro com potencial biotecnológico e agrícola, especialmente visando o controle da antracnose
Beneficiário:Elliot Watanabe Kitajima
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 11/18740-5 - Detecção e caracterização de quitinases em bibliotecas metagenômicas de manguezais do Estado de São Paulo
Beneficiário:Joelma Marcon
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 10/50445-0 - Estado da variabilidade genética de Puccinia psidiiwinter agente causal de ferrugem em Eucalyptus spp
Beneficiário:Maria Carolina Quecine Verdi
Linha de fomento: Bolsas no Brasil - Pós-Doutorado