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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Blood group antigen studies using CdTe quantum dots and flow cytometry

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Autor(es):
Cabral Filho, Paulo E. [1] ; Pereira, Maria I. A. [1] ; Fernandes, Heloise P. [2] ; de Thomaz, Andre A. [3] ; Cesar, Carlos L. [3] ; Santos, Beate S. [4] ; Barjas-Castro, Maria L. [2] ; Fontes, Adriana [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Fed Pernambuco, Dept Biofis & Radiobiol, Recife, PE - Brazil
[2] Univ Estadual Campinas, Inst Nacl Ciencia & Tecnol Sangue, Ctr Hematol & Hemoterapia, Campinas, SP - Brazil
[3] Univ Estadual Campinas, Inst Fis Gleb Wataghin, Dept Eletron Quant, Campinas, SP - Brazil
[4] Univ Fed Pernambuco, Dept Ciencias Farmaceut, Recife, PE - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo de Revisão
Fonte: INTERNATIONAL JOURNAL OF NANOMEDICINE; v. 10, p. 4393-4404, 2015.
Citações Web of Science: 12
Resumo

New methods of analysis involving semiconductor nanocrystals (quantum dots {[}QDs]) as fluorescent probes have been highlighted in life science. QDs present some advantages when compared to organic dyes, such as size-tunable emission spectra, broad absorption bands, and principally exceptional resistance to photobleaching. Methods applying QDs can be simple, not laborious, and can present high sensibility, allowing biomolecule identification and quantification with high specificity. In this context, the aim of this work was to apply dual-color CdTe QDs to quantify red blood cell (RBC) antigen expression on cell surface by flow cytometric analysis. QDs were conjugated to anti-A or anti-B monoclonal antibodies, as well as to the anti-H (Ulex europaeus I) lectin, to investigate RBCs of A(1), B, A(1)B, O, A(2), and A(weak) donors. Bioconjugates were capable of distinguishing the different expressions of RBC antigens, both by labeling efficiency and by flow cytometry histogram profile. Furthermore, results showed that RBCs from Aweak donors present fewer amounts of A antigens and higher amounts of H, when compared to A(1) RBCs. In the A group, the amount of A antigens decreased as A(1) > A(3) > A(X) = A(el), while H antigens were A(X) = A(el) > A(1). Bioconjugates presented stability and remained active for at least 6 months. In conclusion, this methodology with high sensibility and specificity can be applied to study a variety of RBC antigens, and, as a quantitative tool, can help in achieving a better comprehension of the antigen expression patterns on RBC membranes. (AU)

Processo FAPESP: 08/57906-3 - Instituto Nacional de Fotônica Aplicada à Biologia Celular - INFABIC
Beneficiário:Hernandes Faustino de Carvalho
Linha de fomento: Auxílio à Pesquisa - Temático