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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

High current density PQQ-dependent alcohol and aldehyde dehydrogenase bioanodes

Texto completo
Autor(es):
Aquino Neto, Sidney [1, 2, 3] ; Hickey, David P. [1] ; Milton, Ross D. [2, 3] ; De Andrade, Adalgisa R. [1] ; Minteer, Shelley D. [2, 3]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Quim, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-14040901 Ribeirao Preto, SP - Brazil
[2] Univ Utah, Dept Chem, Salt Lake City, UT 84112 - USA
[3] Univ Utah, Dept Mat Sci & Engn, Salt Lake City, UT 84112 - USA
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: BIOSENSORS & BIOELECTRONICS; v. 72, p. 247-254, OCT 15 2015.
Citações Web of Science: 16
Resumo

In this paper, we explore the bioelectrooxidation of ethanol using pyrroloquinoline quinone (PQQ)-dependent alcohol and aldehyde dehydrogenase (ADH and AldDH) enzymes for biofuel cell applications. The bioanode architectures were designed with both direct electron transfer (DET) and mediated electron transfer (MET) mechanisms employing high surface area materials such as multi-walled carbon nanotubes (MWCNTs) and MWCNT-decorated gold nanoparticles, along with different immobilization techniques. Three different polymeric matrices were tested (tetrabutyl ammonium bromide (TBAB)modified Nafion; octyl-modified linear polyethyleneimine (C-8-LPEI); and cellulose) in the DET studies. The modified Nafion membrane provided the best electrical communication between enzymes and the electrode surface, with catalytic currents as high as 16.8 +/- 2.1 mu A cm(-2). Then, a series of ferrocene redox polymers were evaluated for MET. The redox polymer 1,1'-dimethylferrocene-modified linear polyethyleneimine (FcMe(2)-C-3-LPEI) provided the best electrochemical response. Using this polymer, the electrochemical assays conducted in the presence of MWCNTs and MWCNTs-Au indicated a J(max) of 781 +/- 59 mu A cm(-2) and 925 +/- 68 mu A cm(-2), respectively. Overall, from the results obtained here, DET using the PQQ-dependent ADH and AldDH still lacks high current density, while the bioanodes that operate via MET employing ferrocene-modified LPEI redox polymers show efficient energy conversion capability in ethanol/air biofuel cells. (C) 2015 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 12/10667-0 - Preparação de nanocatalisadores híbridos contendo enzimas, nanotubos de carbono e nanopartículas metálicas para biocélula a combustível EtOH/O2
Beneficiário:Sidney de Aquino Neto
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 14/00536-0 - Preparação de nanocatalisadores híbridos contendo enzimas, nanotubos de carbono, e nanopartículas metálicas para biocélula a combustível com transferência eletrônica direta utilizando enzimas PQQ-dependentes
Beneficiário:Sidney de Aquino Neto
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado