Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

ATR-FTIR Spectroscopy for the Assessment of Biochemical Changes in Skin Due to Cutaneous Squamous Cell Carcinoma

Texto completo
Autor(es):
Lima, Cassio A. [1] ; Goulart, Viviane P. [1] ; Correa, Luciana [2] ; Pereira, Thiago M. [1, 3] ; Zezell, Denise M. [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] IPEN CNEN SP, Ctr Lasers & Applicat, Inst Pesquisas Energet & Nucl, BR-05508000 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Sch Dent, BR-05508000 Sao Paulo, SP - Brazil
[3] Univ Fed Sao Paulo, ICT, BR-1223128 Sao Jose Dos Campos, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 16, n. 4, p. 6621-6630, APR 2015.
Citações Web of Science: 20
Resumo

Nonmelanoma skin cancers represent 95% of cutaneous neoplasms. Among them, squamous cell carcinoma (SCC) is the more aggressive form and shows a pattern of possible metastatic profile. In this work, we used Fourier transform infrared spectroscopy (FTIR) spectroscopy to assess the biochemical changes in normal skin caused by squamous cell carcinoma induced by multi-stage chemical carcinogenesis in mice. Changes in the absorption intensities and shifts were observed in the vibrational modes associated to proteins, indicating changes in secondary conformation in the neoplastic tissue. Hierarchical cluster analysis was performed to evaluate the potential of the technique to differentiate the spectra of neoplastic and normal skin tissue, so that the accuracy obtained for this classification was 86.4%. In this sense, attenuated total reflection (ATR)-FTIR spectroscopy provides a useful tool to complement histopathological analysis in the clinical routine for the diagnosis of cutaneous squamous cell carcinoma. (AU)

Processo FAPESP: 05/51689-2 - Centro de Pesquisa em Óptica e Fotônica - UNICAMP (CEPOF-UNICAMP)
Beneficiário:Hugo Luis Fragnito
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs