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Genome-wide association study for identifying agronomic candidate loci in common bean

Grant number: 18/15526-1
Support type:Scholarships abroad - Research Internship - Master's degree
Effective date (Start): January 14, 2019
Effective date (End): June 28, 2019
Field of knowledge:Agronomical Sciences - Agronomy
Cooperation agreement: Network of Italian Universities
Principal Investigator:Luciana Lasry Benchimol-Reis
Grantee:Caléo Panhoca de Almeida
Supervisor abroad: Roberto Papa
Home Institution: Instituto Agronômico (IAC). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). Campinas , SP, Brazil
Local de pesquisa : Università Politecnica delle Marche (UNIVPM), Italy  
Associated to the scholarship:17/01753-3 - Marker assisted selection and genotyping by sequenecing (GBS) toward angular leaf resistance in common beans, BP.MS

Abstract

The common bean (Phaseolus vulgaris L.) is the main source of vegetable protein in the human diet and due to its nutritional characteristics, it is a legume of great importance worldwide. With the advance of technology and the fall in the cost of new generation sequencing (NGS), obtaining genotypic data such as SNPs is becoming more and more accessible. The main "bottleneck" today is the exploitation of the large amount of data obtained, so that the investment for genotyping can be fully utilized. Genome wide association studies (GWAS) allow the identification of loci that influence the expression mainly of quantitative characteristics. In this context, the current project aims to identify QTLs/genes associated with color control, brightness and darkening of the grain, flowering cycle, maturation and protein content. For this reason, a set of 5,679 GBS genotyped SNPs will be used in a population composed of 91 backcrossing inbred lines and 6,000 SNPs genotyped via BeadChip in a core collection composed of 288 accessions from the Agronomic Institute of Campinas (IAC), in a total of 379 Brazilian accessions with 11,679 SNPs. The work will allow the integration of the genotypic data of the Brazilian accessions to the BEAN_ADAPT project, contributing significantly to the understanding of evolution, genetic diversity and population structure, making possible the application of the results in future programs of common bean breeding in the world.